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Nuclear Factor-κB Regulates Inflammatory Cell Apoptosis and Phagocytosis in Rat Carrageenin-Sponge Implant Model
- Source :
- Scopus-Elsevier
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- In the present study we investigated whether apoptosis and phagocytosis are regulated by nuclear factor (NF)-kB in a model of chronic inflammation. The subcutaneous implant of carrageenin-soaked sponges elicited an inflammatory response, characterized by a time-related increase of leukocyte infiltration into the sponge and tissue formation, which was inhibited by simultaneous injection of wild-type oligodeoxynucleotide decoy to NF-kB. Molecular and morphological analysis performed on infiltrated cells demonstrated: 1) an inhibition of NF-kB/DNA binding activity; 2) an increase of polymorphonuclear leukocyte apoptosis correlated either to an increase of p53 or Bax and decrease of Bcl-2 protein expression; and 3) an increase of phagocytosis of apoptotic polymorphonuclear leukocytes by macrophages associated with an increase of transforming growth factor-beta and decrease of tumor necrosis factor- alpha as well as nitrite/ nitrate production. Our results, showing that blockade of NF-kB by oligodeoxynucleotide decoy increases inflammatory cell apoptosis and phagocytosis, may contribute to lead to new insights into the mechanisms governing the inflammatory process. (Am J Pathol 2004, 165:115–126)
- Subjects :
- Male
Time Factors
Neutrophils
Phagocytosis
Blotting, Western
Oligonucleotides
Apoptosis
Enzyme-Linked Immunosorbent Assay
Inflammation
Carrageenan
Pathology and Forensic Medicine
Flow cytometry
Transforming Growth Factor beta1
Cell Movement
Transforming Growth Factor beta
medicine
Animals
Rats, Wistar
Transcription factor
Nitrites
Nitrates
biology
medicine.diagnostic_test
NF-kappa B
Transforming growth factor beta
Flow Cytometry
NFKB1
Immunohistochemistry
Rats
Cell biology
Disease Models, Animal
Immunology
biology.protein
Tumor necrosis factor alpha
medicine.symptom
Transcription Factors
Regular Articles
Subjects
Details
- ISSN :
- 00029440
- Volume :
- 165
- Database :
- OpenAIRE
- Journal :
- The American Journal of Pathology
- Accession number :
- edsair.doi.dedup.....c8c1777be67de514e85ce3b139cb1cfa