Back to Search
Start Over
New Intracellular Shikimic Acid Biosensor for Monitoring Shikimate Synthesis in Corynebacterium glutamicum
- Source :
- ACS synthetic biology. 7(2)
- Publication Year :
- 2017
-
Abstract
- The quantitative monitoring of intracellular metabolites with in vivo biosensors provides an efficient means of identifying high-yield strains and observing product accumulation in real time. In this study, a shikimic acid (SA) biosensor was constructed from a LysR-type transcriptional regulator (ShiR) of Corynebacterium glutamicum. The SA biosensor specifically responded to the increase of intracellular SA concentration over a linear range of 19.5 ± 3.6 to 120.9 ± 1.2 fmole at the single-cell level. This new SA biosensor was successfully used to (1) monitor the SA production of different C. glutamicum strains; (2) develop a novel result-oriented high-throughput ribosome binding site screening and sorting strategy that was used for engineering high-yield shikimate-producing strains; and (3) engineer a whole-cell biosensor through the coexpression of the SA sensor and a shikimate transporter shiA gene in C. glutamicum RES167. This work demonstrated that a new intracellular SA biosensor is a valuable tool facilitating the fast development of microbial SA producer.
- Subjects :
- 0301 basic medicine
High-throughput screening
030106 microbiology
Biomedical Engineering
Shikimic Acid
macromolecular substances
Biosensing Techniques
Biology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Corynebacterium glutamicum
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
In vivo
technology, industry, and agriculture
Transporter
General Medicine
Shikimic acid
Ribosomal binding site
Biochemistry
chemistry
bacteria
Biosensor
Intracellular
Transcription Factors
Subjects
Details
- ISSN :
- 21615063
- Volume :
- 7
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- ACS synthetic biology
- Accession number :
- edsair.doi.dedup.....c8e14cce99e340151fc6454a817c9566