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Effects of dietary antioxidants and 2-amino-3-methylimidazo[4,5-f]- quinoline (IQ) on preneoplastic lesions and on oxidative damage, hormonal status, and detoxification capacity in the rat
- Source :
- Food and Chemical Toxicology. 41:1315-1323
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- The potential beneficial or adverse affect of prolonged dietary administration of moderate to high doses (1–100 mg/kg diet) of the antioxidants, lycopene, quercetin and resveratrol or a mixture of lycopene and quercetin was investigated in male F344 rats. Selected markers for toxicity and defense mechanisms were assayed in blood, liver and colon and the impact of the antioxidant administrations on putative preneoplastic changes in liver and colon was assessed. The dietary carcinogen, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) (200 mg/kg diet) served as a pro-oxidant, genotoxicity and general toxicity control. IQ increased the levels of protein and DNA oxidation products in plasma, the area of glutathione S-transferase-placental form positive (GST-P) foci in the liver as well as the number of colonic aberrant crypt foci (ACF). All antioxidants and the antioxidant combination significantly increased the level of lymphocytic DNA damage, to an extent comparable with the effect induced by IQ. In contrast to the control group where no GST-P foci were detected, GST-P foci were detected in animals exposed to quercetin, lycopene and the combination of the two. However, the increase in the volume of GST-P foci did not reach statistical significance. The present results indicate that moderate to high doses of common dietary antioxidants can damage lymphocyte DNA and induce low levels of preneoplastic liver lesions in experimental animals. Long-term exposure to moderate to high doses of antioxidants may thus via pro-oxidative mechanisms and non-oxidative mechanisms modulate carcinogenesis.
- Subjects :
- Male
Antioxidant
Colon
DNA damage
Placenta
medicine.medical_treatment
Pharmacology
Biology
Toxicology
medicine.disease_cause
Antioxidants
chemistry.chemical_compound
Lycopene
Stilbenes
medicine
Animals
Carcinogen
Glutathione Transferase
General Medicine
Glutathione
Carotenoids
Immunohistochemistry
Hormones
Rats, Inbred F344
Diet
Rats
Oxidative Stress
Liver
chemistry
Biochemistry
Resveratrol
Toxicity
Quinolines
Quercetin
Comet Assay
Oxidation-Reduction
Precancerous Conditions
Biomarkers
Genotoxicity
Food Science
Aberrant crypt foci
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....c8e6a4ce5f6b0bce82e11edb33217977