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Fungicidal and anti-biofilm activities of trimethylchitosan-stabilized silver nanoparticles against Candida species in zebrafish embryos
- Source :
- International journal of biological macromolecules. 143
- Publication Year :
- 2019
-
Abstract
- Herein, positively surface-charged silver nanoparticles (AgNPs) capped with trimethylchitosan nitrate (TMCN) were synthesized using an environmentally friendly method. Nano-sized TMCN-AgNPs (~80 nm) with high zeta potential (>30 mV) provide sufficient static repulsion to stabilize colloid AgNPs in aqueous solutions without aggregation for >3 months. In in vitro cell cycle assays, TMCN-AgNPs showed low cytotoxicity towards L929 cells. A microdilution inhibition assay demonstrated the antifungal potential of TMCN-AgNPs, with a minimum inhibitory concentration of 0.06 mM against Candida tropicalis ATCC 750, and 0.46 mM against both Candida albicans ATCC 76615 and Candida glabrata ATCC 15545. Moreover, the addition of TMCN-AgNPs at 0.23 mM significantly reduced biofilm formation in 96-well plates with C. albicans and C. tropicalis. Importantly, when zebrafish eggs were infected with Candida cells, 0.23 mM TMCN-AgNPs greatly diminished the amount of biofilm on eggs and rescued the survival of embryos by up to 70%.
- Subjects :
- Antifungal Agents
Embryo, Nonmammalian
Silver
Metal Nanoparticles
02 engineering and technology
Microbial Sensitivity Tests
Biochemistry
Silver nanoparticle
Microbiology
Cell Line
Candida tropicalis
03 medical and health sciences
Minimum inhibitory concentration
Mice
Structural Biology
Animals
Cytotoxicity
Candida albicans
Molecular Biology
Zebrafish
030304 developmental biology
Candida
Ovum
0303 health sciences
Chitosan
biology
Candida glabrata
Cell Death
Chemistry
Biofilm
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Corpus albicans
Biofilms
0210 nano-technology
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....c92efc83fdaafd120ac61866ed44fa43