Back to Search
Start Over
Antioxidant Activities of Trypsin Inhibitor, a 33 KDa Root Storage Protein of Sweet Potato (Ipomoea batatas (L.) Lam cv. Tainong 57)
- Source :
- Journal of Agricultural and Food Chemistry. 49:2978-2981
- Publication Year :
- 2001
- Publisher :
- American Chemical Society (ACS), 2001.
-
Abstract
- Trypsin inhibitors (TIs), root storage proteins, were purified from sweet potato (Ipomoea batatas[L.] Lam cv. Tainong 57) roots by trypsin affinity column according to the methods of Hou and Lin (Plant Sci. 1997, 126, 11-19 and Plant Sci. 1997, 128, 151-158). A single band of 33 kDa TI was obtained by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. This purified 33 kDa TI had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. There was positive correlation between scavenging effects against DPPH (2 to 22%) and amounts of 33 kDa TI (1.92 to 46 pmol). The scavenging activities of 33 kDa TI against DPPH were calculated from linear regression to be about one-third of those of glutathione between 5 and 80 pmol. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detection, it was found that 33 kDa TI could capture hydroxyl radical, and the intensities of EPR signal were significantly decreased from 1.5 to 6 pmol of 33 kDa TI compared to those of the controls. It is suggested that 33 kDa TI, one of the sweet potato root storage proteins, may play a role as an antioxidant in roots and may be beneficial to health when it is consumed.
- Subjects :
- DPPH
Trypsin inhibitor
Ipomoea
Antioxidants
chemistry.chemical_compound
medicine
Storage protein
Solanaceae
Plant Proteins
Gel electrophoresis
chemistry.chemical_classification
Chromatography
biology
Hydroxyl Radical
Electron Spin Resonance Spectroscopy
Free Radical Scavengers
General Chemistry
biology.organism_classification
Trypsin
Molecular Weight
chemistry
Biochemistry
Plant protein
Electrophoresis, Polyacrylamide Gel
Hydroxyl radical
alpha-Amylases
Trypsin Inhibitors
General Agricultural and Biological Sciences
medicine.drug
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....c934024ab1a584aa11ee72e7151b61b3