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Anti-influenza virus phytochemicals from Radix Paeoniae Alba and characterization of their neuraminidase inhibitory activities
- Source :
- Journal of ethnopharmacology. 253
- Publication Year :
- 2019
-
Abstract
- Ethnopharmacological relevance Bai Shao (Radix Paeoniae Alba, BS), the root of Paeonia lactiflora Pall., in ancient China was used for Wen Bing (Warm Disease) treatment. Wen Bing has the symptoms of influenza. Ethanol extract of the root has recently been shown to possess anti-influenza activity. However, the active compounds have not yet been identified. Aim We showed that BS aqueous extract was potent in inhibiting influenza A virus in infected cells. We aimed to isolate the bioactive compounds and characterize the anti-influenza mechanism. Materials and methods Plaque reduction assay was performed for fractions isolated from BS. Hemagglutination inhibition assay and neuraminidase inhibition assay were performed to find the target protein. Molecular docking and reverse genetics were used to confirm the action site of gallic acid on the neuraminidase protein. Results We identified three tannin compounds gallic acid (GA), methyl gallate (MG) and pentagalloylglucose (PGG) in BS aqueous extract that could inhibit the replication of influenza A virus in MDCK cells. While only PGG was found to inhibit the influenza virus-induced hemagglutination of chicken erythrocytes, all three compounds significantly reduced the activity of the neuraminidase. The results from molecular docking and reverse genetics showed that GA interacted with Arg152 of neuraminidase protein. Conclusion Three compounds GA, MG and PGG isolated from BS were found to inhibit influenza A virus in MDCK cells. GA interacts with amino acid Arg152 of the viral neuraminidase. Our study identified anti-influenza compounds of BS and demonstrated their antiviral mechanism, thus providing scientific evidence for using this herb for clinical treatment.
- Subjects :
- Hemagglutination
Phytochemicals
Neuraminidase
medicine.disease_cause
Paeonia
Antiviral Agents
Virus
Madin Darby Canine Kidney Cells
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Dogs
Influenza A Virus, H1N1 Subtype
Gallic Acid
Drug Discovery
Influenza A virus
medicine
Viral neuraminidase
Animals
Gallic acid
Methyl gallate
030304 developmental biology
Pharmacology
0303 health sciences
Hemagglutination assay
biology
Hemagglutination Inhibition Tests
Hydrolyzable Tannins
Molecular Docking Simulation
chemistry
Biochemistry
030220 oncology & carcinogenesis
biology.protein
Subjects
Details
- ISSN :
- 18727573
- Volume :
- 253
- Database :
- OpenAIRE
- Journal :
- Journal of ethnopharmacology
- Accession number :
- edsair.doi.dedup.....c977eb8bf49b292036d0bd0ac93f629d