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miR‑660‑5p is associated with cell migration, invasion, proliferation and apoptosis in renal cell carcinoma
- Source :
- Molecular Medicine Reports.
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system with poor prognosis. microRNAs (miRNAs) are a class of small, non‑coding RNA molecules that serve important roles in biological and pathological processes in several types of human tumors. miRNA (miR)‑660‑5p is dysregulated in many human cancers; however, its role in renal cell carcinoma is currently unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction was performed to examine the expression levels of miR‑660‑5p in RCC tissues and paired normal adjacent tissues (NATs). To determine the function of miR‑660‑5p in RCC cells, wound‑healing and Matrigel assays were performed to determine the effects of miR‑660‑5p on cell migration and invasion, respectively. MTT and Cell Counting kit‑8 assays were performed to determine the effects of miR‑660‑5p on RCC cell proliferation. In addition, flow cytometric analysis was performed to validate the effects of miR‑660‑5p on apoptosis. The results indicated that miR‑660‑5p expression was downregulated in RCC tissues compared with NATs. Restoration of miR‑660‑5p expression using synthetic mimics may suppress cell migration, invasion and proliferation, and induce cell apoptosis, while using synthetic inhibitors may promote cell migration, invasion and proliferation, and suppress cell apoptosis. These results suggested that miR‑660‑5p may serve a tumor suppressive role in RCC tumorigenesis.
- Subjects :
- Adult
Male
0301 basic medicine
Cancer Research
Cell
Apoptosis
Biology
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line, Tumor
microRNA
Genetics
medicine
Humans
Neoplasm Invasiveness
Carcinoma, Renal Cell
Molecular Biology
Aged
Cell Proliferation
Matrigel
Cell growth
Cell migration
Middle Aged
Cell cycle
Kidney Neoplasms
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Female
Carcinogenesis
A431 cells
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....c97f9b1f3c2310721476fff33857d61d