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HOE-BAY 946 has distinct effects on spontaneous and FGF induced proliferation of epithelial and neuroblastoma cell lines
- Source :
- Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft. 176(1)
- Publication Year :
- 1994
-
Abstract
- Summary We have studied in vitro the effects of HOE-BAY 946, a specific inhibitor of tyrosine kinase C, on spontaneous and basic FGF-induced proliferation of the epithelial cell lines A549 and HELA as well as the neuroblastoma cell line SY5Y using a fully automated colorimetric assay for determinations of cell numbers. HOE-BAY suppressed spontaneous proliferation of the epithelial lines, but not the neuroblastoma line, both in the presence and absence of serum. In contrast, the drug completely abolished the mitogenic effect of basic FGF on all cell lines. This suggests an involvement of a tyrosine kinase C activity in the transduction of the FGF signal both in the epithelial and neuroblastoma lines. Since the action of FGF on non-transformed neuronal cells is accompanied by a down-regulation of kinase C, and kinase C activation blocks the FGF effects, our data indicate that differentiative and mitogenic effects of FGF on neuronal cells may employ different transduction mechanisms.
- Subjects :
- Cell
Fibroblast growth factor
Antiviral Agents
Epithelium
Cell Line
HeLa
Transduction (genetics)
Neuroblastoma
Polysaccharides
medicine
Tumor Cells, Cultured
Humans
Drug Interactions
Protein Kinase C
Pentosan Sulfuric Polyester
Analysis of Variance
biology
Kinase
Epithelial Cells
General Medicine
biology.organism_classification
medicine.disease
Molecular biology
Recombinant Proteins
Cell biology
Kinetics
medicine.anatomical_structure
Cell culture
Fibroblast Growth Factor 2
Anatomy
Tyrosine kinase
Cell Division
Developmental Biology
HeLa Cells
Subjects
Details
- ISSN :
- 09409602
- Volume :
- 176
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
- Accession number :
- edsair.doi.dedup.....c98be74c7016d76a8f5dceeef430d79a