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Stimulation of Glucose Transport in Cultures of Density-Inhibited Chick Embryo Cells

Authors :
Bartholomew M. Sefton
Harry Rubin
Source :
Proceedings of the National Academy of Sciences. 68:3154-3157
Publication Year :
1971
Publisher :
Proceedings of the National Academy of Sciences, 1971.

Abstract

The rate of glucose transport in sparse, rapidly growing chick-embryo fibroblasts is much greater than that in density-inhibited cells. The addition of fresh chicken serum or trypsin to the medium of density-inhibited cells causes a large increase in the rate of glucose transport that is detectable 15 min after addition. The increase in glucose transport precedes the increase in DNA synthesis by 5-6 hr. Only small changes in rates of transport are seen with nucleosides or a nonmetabolizable amino acid. The increase in glucose transport requires protein synthesis but not RNA or DNA synthesis.

Details

ISSN :
10916490 and 00278424
Volume :
68
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences
Accession number :
edsair.doi.dedup.....c98eb199e00da09cc27284d310e1b408