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High-throughput quantitative analysis of phytohormones in sorghum leaf and root tissue by ultra-performance liquid chromatography-mass spectrometry
- Source :
- Analytical and Bioanalytical Chemistry. 411:4839-4848
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Plant development, growth, and adaptation to stress are regulated by phytohormones, which can influence physiology even at low concentrations. Phytohormones are chemically grouped according to both structure and function as auxins, cytokinins, abscisic acid, jasmonates, salicylates, gibberellins, and brassinosteroids, among others. This chemical diversity and requirement for highly sensitive detection in complex matrices create unique challenges for comprehensive phytohormone analysis. Here, we present a robust and efficient quantitative UPLC-MS/MS assay for 17 phytohormones, including jasmonates, salicylates, abscisic acid, gibberellins, cytokinins, and auxins. Using this assay, 12 phytohormones were detected and quantified in sorghum plant tissue without the need for solid phase extraction (SPE) or liquid-liquid extraction. Variation of phytohormone profiles was explored in both root and leaf tissues between three genotypes, harvested at two different developmental time points. The results highlight the importance of tissue type, sampling time, and genetic factors when designing experiments that involve phytohormone analysis of sorghum. This research lays the groundwork for future studies, which can combine phytohormone profiling with other datasets such as transcriptome, soil microbiome, genome, and metabolome data, to provide important functional information about adaptation to stress and other environmental variables.
- Subjects :
- 02 engineering and technology
Biology
Plant Roots
01 natural sciences
Biochemistry
Analytical Chemistry
Transcriptome
chemistry.chemical_compound
Plant Growth Regulators
Tandem Mass Spectrometry
Auxin
Liquid chromatography–mass spectrometry
Metabolome
Solid phase extraction
Abscisic acid
Chromatography, High Pressure Liquid
Sorghum
chemistry.chemical_classification
fungi
010401 analytical chemistry
food and beverages
021001 nanoscience & nanotechnology
biology.organism_classification
High-Throughput Screening Assays
0104 chemical sciences
Plant Leaves
chemistry
Gibberellin
0210 nano-technology
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 411
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....c9a56c786e59f64b9bd501d0b0489b11