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Induction and Exhaustion of Lymphocytic Choriomeningitis Virus–specific Cytotoxic T Lymphocytes Visualized Using Soluble Tetrameric Major Histocompatibility Complex Class I–Peptide Complexes
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1998
- Publisher :
- Rockefeller University Press, 1998.
-
Abstract
- This study describes the construction of soluble major histocompatibility complexes consisting of the mouse class I molecule, H-2Db, chemically biotinylated β2 microglobulin and a peptide epitope derived from the glycoprotein (GP; amino acids 33–41) of lymphocytic choriomeningitis virus (LCMV). Tetrameric class I complexes, which were produced by mixing the class I complexes with phycoerythrin-labeled neutravidin, permitted direct analysis of virus-specific cytotoxic T lymphocytes (CTLs) by flow cytometry. This technique was validated by (a) staining CD8+ cells in the spleens of transgenic mice that express a T cell receptor (TCR) specific for H-2Db in association with peptide GP33–41, and (b) by staining virus-specific CTLs in the cerebrospinal fluid of C57BL/6 (B6) mice that had been infected intracranially with LCMV-DOCILE. Staining of spleen cells isolated from B6 mice revealed that up to 40% of CD8+ T cells were GP33 tetramer+ during the initial phase of LCMV infection. In contrast, GP33 tetramers did not stain CD8+ T cells isolated from the spleens of B6 mice that had been infected 2 mo previously with LCMV above the background levels found in naive mice. The fate of virus-specific CTLs was analyzed during the acute phase of infection in mice challenged both intracranially and intravenously with a high or low dose of LCMV-DOCILE. The results of the study show that the outcome of infection by LCMV is determined by antigen load alone. Furthermore, the data indicate that deletion of virus-specific CTLs in the presence of excessive antigen is preceded by TCR downregulation and is dependent upon perforin.
- Subjects :
- Pore Forming Cytotoxic Proteins
Virus-specific Cytotoxic T-lymphocytes
Protein Conformation
Immunology
Receptors, Antigen, T-Cell
Mice, Transgenic
chemical and pharmacologic phenomena
CD8-Positive T-Lymphocytes
Major histocompatibility complex
Lymphocytic choriomeningitis
Article
Epitope
Major Histocompatibility Complex
Interferon-gamma
Mice
03 medical and health sciences
0302 clinical medicine
Antigen
medicine
Animals
Lymphocytic choriomeningitis virus
Immunology and Allergy
Cytotoxic T cell
Glycoproteins
030304 developmental biology
0303 health sciences
Membrane Glycoproteins
biology
Perforin
Histocompatibility Antigens Class I
T-cell receptor
hemic and immune systems
Articles
Flow Cytometry
medicine.disease
Virology
Molecular biology
3. Good health
biology.protein
Peptides
beta 2-Microglobulin
Spleen
CD8
T-Lymphocytes, Cytotoxic
030215 immunology
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....c9b0d75b0004eea63d536d87f3678309
- Full Text :
- https://doi.org/10.1084/jem.187.9.1383