Back to Search
Start Over
Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network
- Source :
- Journal of Animal Science and Biotechnology, Journal of Animal Science and Biotechnology, Vol 9, Iss 1, Pp 1-11 (2018)
- Publication Year :
- 2017
-
Abstract
- Background Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development. The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes, amphibians, and mammals have been elucidated. There are also species-specific maternally inherited transcriptional factors and their intricate transcriptional networks important in the acquisition of pluripotency. In avian species, however, the core transcriptional network that governs the acquisition of pluripotency during early embryonic development is not well understood. Results We found that chicken NANOG (cNANOG) was expressed in the stages between the pre-ovulatory follicle and oocyte and was continuously detected in Eyal-Giladi and Kochav stage I (EGK.I) to X. However, cPOUV was not expressed during folliculogenesis, but began to be detectable between EGK.V and VI. Unexpectedly, cSOX2 could not be detected during folliculogenesis and intrauterine embryonic development. Instead of cSOX2, cSOX3 was maternally inherited and continuously expressed during chicken intrauterine development. In addition, we found that the pluripotency-related genes such as cENS-1, cKIT, cLIN28A, cMYC, cPRDM14, and cSALL4 began to be dramatically upregulated between EGK.VI and VIII. Conclusion These results suggest that chickens have a unique pluripotent circuitry since maternally inherited cNANOG and cSOX3 may play an important role in the initial acquisition of pluripotency. Moreover, the acquisition of pluripotency in chicken embryos occurs at around EGK.VI to VIII.
- Subjects :
- 0301 basic medicine
Homeobox protein NANOG
Pluripotency
Avian
Biology
Cell fate determination
Biochemistry
03 medical and health sciences
medicine
Gene
Transcription factor
lcsh:SF1-1100
lcsh:Veterinary medicine
030102 biochemistry & molecular biology
Research
Embryogenesis
Embryo
Oocyte
Cell biology
030104 developmental biology
medicine.anatomical_structure
NANOG
Embryonic development
Transcriptional factor
lcsh:SF600-1100
Animal Science and Zoology
lcsh:Animal culture
Folliculogenesis
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 16749782
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of animal science and biotechnology
- Accession number :
- edsair.doi.dedup.....c9b13e839c97d71578a24e74f6d4bc20