Back to Search
Start Over
DNA-based stable isotope probing: a link between community structure and function
DNA-based stable isotope probing: a link between community structure and function
- Source :
- The Science of the total environment. 407(12)
- Publication Year :
- 2008
-
Abstract
- DNA-based molecular techniques permit the comprehensive determination of microbial diversity but generally do not reveal the relationship between the identity and the function of microorganisms. The first direct molecular technique to enable the linkage of phylogeny with function is DNA-based stable isotope probing (DNA-SIP). Applying this method first helped describe the utilization of simple compounds, such as methane, methanol or glucose and has since been used to detect microbial communities active in the utilization of a wide variety of compounds, including various xenobiotics. The principle of the method lies in providing (13)C-labeled substrate to a microbial community and subsequent analyses of the (13)C-DNA isolated from the community. Isopycnic centrifugation permits separating (13)C-labeled DNA of organisms that utilized the substrate from (12)C-DNA of the inactive majority. As the whole metagenome of active populations is isolated, its follow-up analysis provides successful taxonomic identification as well as the potential for functional gene analyses. Because of its power, DNA-SIP has become one of the leading techniques of microbial ecology research. But from other point of view, it is a labor-intensive method that requires careful attention to detail during each experimental step in order to avoid misinterpretation of results.
- Subjects :
- DNA, Bacterial
Carbon Isotopes
Environmental Engineering
Bacteria
Stable isotope ratio
Stable-isotope probing
Computational biology
Biology
Pollution
chemistry.chemical_compound
Biodegradation, Environmental
chemistry
Microbial ecology
Microbial population biology
Biochemistry
Metagenomics
Phylogenetics
Isotope Labeling
Environmental Chemistry
Identification (biology)
Waste Management and Disposal
DNA
Ecosystem
Subjects
Details
- ISSN :
- 00489697
- Volume :
- 407
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The Science of the total environment
- Accession number :
- edsair.doi.dedup.....c9ec54185eab38db4db466b5a14c87ea