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The long noncoding RNA NARL regulates immune responses via microRNA-mediated NOD1 downregulation in teleost fish
- Source :
- The Journal of Biological Chemistry
- Publication Year :
- 2020
-
Abstract
- Increasing evidence shows that the long noncoding RNA (lncRNA) is a major regulator and participates in the regulation of various physiological and pathological processes, such as cell proliferation, differentiation, metastasis, and apoptosis. Unlike mammals, however, the study of lncRNA in lower invertebrates is just beginning and the extent of lncRNA-mediate regulation remains unclear. Here, we for the first time identify an lncRNA, termed nucleotide oligomerization domain 1 (NOD1) antibacterial and antiviral-related lncRNA (NARL), as a key regulator for innate immunity in teleost fish. We found that NOD1 plays an important role in the antibacterial and antiviral process in fish and that the microRNA miR-217-5p inhibits NOD1 expression and thus weakens the NF-κB and the IRF3-driven signaling pathway. Furthermore, our results indicated that NARL functions as a competing endogenous RNA (ceRNA) for miR-217-5p to regulate protein abundance of NOD1; thus, invading microorganisms are eliminated and immune responses are promoted. Our study also demonstrates the regulation mechanism that lncRNA NARL can competitive adsorption miR-217-5p to regulate the miR-217-5p/NOD1 axis is widespread in teleost fish. Taken together, our results reveal that NARL in fish is a critical positive regulator of innate immune responses to viral and bacterial infection by suppressing a feedback to NOD1-NF-κB/IRF3-mediated signaling.
- Subjects :
- 0301 basic medicine
PRR, pattern recognition receptor
Regulator
Gene Expression
Apoptosis
MDP, muramyl dipeptide
Biochemistry
chemistry.chemical_compound
Nod1 Signaling Adaptor Protein
ceRNA, competing endogenous RNA
NOD1
NLR, nucleotide-binding oligomerization domain-like receptor
microRNA
Pattern recognition receptor
NF-kappa B
RIP, RNA immunoprecipitation
Cell biology
RNA, Long Noncoding
MIC, M. miiuy intestine cell
Signal Transduction
Research Article
China
NARL, NOD1 antibacterial and antiviral-related lncRNA
NF-κB, nuclear factor-κB
Biology
03 medical and health sciences
Downregulation and upregulation
EPC, Epithelioma papulosum cyprinid
Animals
long noncoding RNA
Molecular Biology
Cell Proliferation
Innate immune system
030102 biochemistry & molecular biology
Competing endogenous RNA
NOD1, nucleotide oligomerization domain 1
NF-κB
Cell Biology
ceRNA
Immunity, Innate
immune responses
Perciformes
body regions
MicroRNAs
030104 developmental biology
chemistry
Gene Expression Regulation
bacteria
LPS, lipopolysaccharides
SCRV, Siniperca chuatsi rhabdovirus
Interferon Regulatory Factor-3
lncRNA, long noncoding RNA
MAPK, mitogen-activated protein kinase
Subjects
Details
- ISSN :
- 1083351X
- Volume :
- 296
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....c9fac645ca72615a90187f4787db1734