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Dual photonic bandgap hollow sphere colloidal photonic crystals for real-time fluorescence enhancement in living cells

Dual photonic bandgap hollow sphere colloidal photonic crystals for real-time fluorescence enhancement in living cells

Authors :
Olivier Deschaume
Stijn Jooken
Yovan de Coene
Atsushi Yamada
Koen Clays
Kuo Zhong
Carmen Bartic
Wei Yu
Olga Krylychkina
Source :
Biosensorsbioelectronics. 194
Publication Year :
2021

Abstract

To overcome the problems of refractive index matching and increased disorder when working with traditional heterostructure colloidal photonic crystals (CPCs) with dual or multiple photonic bandgaps (PBGs) for fluorescence enhancement in water, we propose the use of a chemical heterostructure in hollow sphere CPCs (HSCPCs). A partial chemical modification of the HSCPC creates a large contrast in wettability to induce the heterostructure, while the hollow spheres increase the refractive index difference when used in aqueous environment. With the platform, fluorescence enhancement reaches around 160 times in solution, and 72 times (signal-to-background ratio ~7 times) in cells during proof-of-concept live cardiomyocyte contractility experiments. Such photonic platform can be further exploited for chemical sensing, bioassays, and environmental monitoring. Moreover, the introduction of chemical heterostructures provides new design principles for functionalized photonic devices.

Details

ISSN :
18734235
Volume :
194
Database :
OpenAIRE
Journal :
Biosensorsbioelectronics
Accession number :
edsair.doi.dedup.....ca1258d23d310732b483b322a79ad558