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CADM1/TSLC1 inactivation by promoter hypermethylation is a frequent event in colorectal carcinogenesis and correlates with late stages of the disease
- Source :
- International journal of cancer. 128(2)
- Publication Year :
- 2009
-
Abstract
- Cell adhesion molecule 1 (CADM1/TSLC1), a putative tumor suppressor involving in cell adhesion, proliferation and apoptosis, is frequently inactivated in several carcinomas due to promoter hypermethylation. But alterations of CADM1/TSLC1 in colorectal carcinogenesis and clinical significance have not been elucidated yet. The aim of this study was to determine the role of functional inactivation of CADM1/TSLC1 gene in colorectal tumorigenesis and its potential as a novel epigenetic marker for clinical assessment of patients with colorectal cancer. We measured CADM1/TSLC1 expression levels in 8 colorectal cancer cell lines, 54 primary colorectal carcinomas and their corresponding non-cancerous tissues by reverse transcription polymerase chain reaction, western blot analysis and immunohistochemistry. We analyzed CADM1/TSLC1 promoter methylation status by bisulfite genomic sequencing and the methylation special polymerase chain reaction, and evaluated its correlation with clinicopathological characteristics. All statistical tests were 2-sides. Downregulation of CADM1/TSLC1 expression was observed in 7 of 8 (88%) colorectal cancer cell lines and in 39 of 54 (72%) primary colorectal carcinomas. Hypermethylation of CADM1/TSLC1 promoter region occurred in 6 of 8 (75%) colorectal cancer cell lines and 32 of 54 (59%) primary colorectal carcinomas, and was correlated with advanced colorectal carcinoma. Epigenetic inactivation of CADM1/TSLC1 gene is a frequent alteration in development of colorectal cancer and can be a potential biomarker for molecular staging of patients with colorectal cancer.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Tumor suppressor gene
Deleted in Colorectal Cancer
Colorectal cancer
Down-Regulation
Immunoglobulins
Mouse model of colorectal and intestinal cancer
Biology
medicine.disease_cause
Cell Line, Tumor
medicine
Humans
Epigenetics
RNA, Messenger
Promoter Regions, Genetic
Aged
Neoplasm Staging
Tumor Suppressor Proteins
Cell Adhesion Molecule-1
Cancer
DNA Methylation
Middle Aged
medicine.disease
Oncology
DNA methylation
Cancer research
Female
Carcinogenesis
Colorectal Neoplasms
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 10970215
- Volume :
- 128
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International journal of cancer
- Accession number :
- edsair.doi.dedup.....ca42a1f9d586453f8dacfb285fc17925