Back to Search
Start Over
Hemolytic activity and biofilm-formation among clinical isolates of group B streptococcus causing acute urinary tract infection and asymptomatic bacteriuria
- Source :
- International Journal of Medical Microbiology, Vol 311, Iss 6, Pp 151520-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Streptococcus agalactiae, also known as group B Streptococcus, is an aetiological agent of urinary tract infection (UTI) in adults, including cystitis, pyelonephritis and asymptomatic bacteriuria (ABU). Whereas ABU-causing S. agalactiae (ABSA) have been shown to grow and achieve higher culture denstity in human urine compared to uropathogenic S. agalactiae (UPSA) other phenotypic distinctions between S. agalactiae isolated from different forms of UTI are not known. Here, we define the hemolytic activities and biofilm-formation of a collection of clinical isolates of UPSA, ABSA and recurrent S. agalactiae bacteriuria (rSAB) strains to explore these phenotypes in the context of clinical history of isolates. A total of 61 UPSA, 184 ABSA, and 47 rSAB isolates were analyzed for relative hemolytic activity by spot assay on blood agar, which was validated using a erythrocyte lysis suspension assay. Biofilm formation was determined by microtiter plate assay with Lysogeny and Todd-Hewitt broths supplemented with 1% glucose to induce biofilm formation. We also used multiplex PCR to analyze isolates for the presence of genes encoding adhesive pili, which contribute to biofilm formation. Comparing the hemolytic activities of 292 isolates showed, surprisingly, that ABSA strains were significantly more likely to be highly hemolytic compared to other strains. In contrast, there were no differences between the relative abilities of strains from the different clinical history groups to form biofilms. Taken together, these findings demonstrate a propensity of S. agalactiae causing ABU to be highly hemolytic but no link between clinical history of UTI strains and ability to form biofilm.
- Subjects :
- Microbiology (medical)
medicine.medical_specialty
Group B streptococcus
Bacteriuria
Context (language use)
Biology
medicine.disease_cause
Hemolysis
Microbiology
Group B
Streptococcus agalactiae
Agar plate
Other systems of medicine
Medical microbiology
medicine
Humans
Urinary tract infection
Streptococcus
Biofilm
General Medicine
medicine.disease
QR1-502
Infectious Diseases
Biofilms
Urinary Tract Infections
RZ201-999
Subjects
Details
- ISSN :
- 14384221
- Volume :
- 311
- Database :
- OpenAIRE
- Journal :
- International Journal of Medical Microbiology
- Accession number :
- edsair.doi.dedup.....ca6ca97fbd092828310670f522454ecb
- Full Text :
- https://doi.org/10.1016/j.ijmm.2021.151520