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Gintonin influences the morphology and motility of adult brain neurons via LPA receptors
- Source :
- Journal of Ginseng Research, Journal of Ginseng Research, Vol 45, Iss 3, Pp 401-407 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Background Gintonin is an exogenous ginseng-derived G-protein-coupled lysophosphatidic acid (LPA) receptor ligand. LPA induces in vitro morphological changes and migration through neuronal LPA1 receptor. Recently, we reported that systemic administration of gintonin increases blood-brain barrier (BBB) permeability via the paracellular pathway and its binding to brain neurons. However, little is known about the influences of gintonin on in vivo neuron morphology and migration in the brain. Materials and methods We examined the effects of gintonin on in vitro migration and morphology using primary hippocampal neural precursor cells (hNPC) and in vivo effects of gintonin on adult brain neurons using real time microscopic analysis and immunohistochemical analysis to observe the morphological and locational changes induced by gintonin treatment. Results We found that treating hNPCs with gintonin induced morphological changes with a cell rounding following cell aggregation and return to individual neurons with time relapses. However, the in vitro effects of gintonin on hNPCs were blocked by the LPA1/3 receptor antagonist, Ki16425, and Rho kinase inhibitor, Y27632. We also examined the in vivo effects of gintonin on the morphological changes and migration of neurons in adult mouse brains using anti-NeuN and -neurofilament H antibodies. We found that acute intravenous administration of gintonin induced morphological and migrational changes in brain neurons. Gintonin induced some migrations of neurons with shortened neurofilament H in the cortex. The in vivo effects of gintonin were also blocked by Ki16425. Conclusion The present report raises the possibility that gintonin could enter the brain and exert its influences on the migration and morphology of adult mouse brain neurons and possibly explains the therapeutic effects of neurological diseases behind the gintonin administration.
- Subjects :
- 0301 basic medicine
HBSS, Hanks' Balanced Salt Solution
medicine.drug_class
OCT, optimum cutting temperature
Motility
Gintonin
DMSO, dimethyl sulfoxide
Hippocampal formation
Biochemistry, Genetics and Molecular Biology (miscellaneous)
hNPC, hippocampal neural precursor cells
LPA receptors
Adult brain neuron
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Precursor cell
Lysophosphatidic acid
medicine
Receptor
EGF, epidermal growth factor
DMEM, Dulbecco's modified Eagle's medium
NFH, neurofilament H
Chemistry
NECAB1, Neuronal calcium binding proteins 1
Botany
Receptor antagonist
LPA, Lysophatidic Acid
Cell aggregation
Cell biology
BBB, blood brain barrier
bFGF, fibroblast growth factor
Morphology and migration
030104 developmental biology
Complementary and alternative medicine
ROCK, Rho-associated protein kinase
QK1-989
030220 oncology & carcinogenesis
Systemic administration
BSA, bovine serum albumin
FITC, fluorescein isothiocyanate
MEM, Modified Eagle's medium
PFA, paraformaldehyde
Research Article
DAPI, 4′,6-diamidino-2-phenylindole
Biotechnology
Subjects
Details
- ISSN :
- 12268453
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Journal of Ginseng Research
- Accession number :
- edsair.doi.dedup.....ca7b35c04d99638d6b8e1671517e2368