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Development of a Liquid Chromatographic Method for Apomorphine Hydrochloride Quantitation in the Active Pharmaceutical Ingredients and in the Dosage Forms
- Source :
- Volume: 4, Issue: 2 44-50, Eurasian Journal of Biological and Chemical Sciences
- Publication Year :
- 2021
- Publisher :
- Muhammet Dogan, 2021.
-
Abstract
- Apomorphine is currently used as a dopamine agonist to treat advanced Parkinson Disease. In the present study, we developed a sensitive, simple, reliable, and robust HPLC method for quality control of apomorphine hydrochloride in bulk drug and pharmaceutical formulations. C18 column was used for separation. Isocratic elution was performed using mobile phase A: 50 mM potassium dihydrogen phosphate solution (pH:3 with ortho-phosphoric acid), B: acetonitrile in the ratio of 85:15 (A: B), and the mobile-phase flow rate was kept at 1 mL min-1. Analyses were carried out at 272 nm using a UV detector. A perfect linear relationship between peak-area versus drug concentration in the range of 10‑100 μg mL-1 was observed (r2, 0.9999). It has been found that the developed method is sensitive (Detection and quantification limits were determined as 1.3 μg mL-1 and 3.8 μg mL-1, respectively), precise (RSD
Details
- ISSN :
- 26515237
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Eurasian Journal of Biological and Chemical Sciences
- Accession number :
- edsair.doi.dedup.....ca96e9cefbb3ea16a1e8f80c07b59bad