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Micropallet Arrays for the Separation of Single, Adherent Cells
- Source :
- Analytical Chemistry. 79:682-687
- Publication Year :
- 2006
- Publisher :
- American Chemical Society (ACS), 2006.
-
Abstract
- The selection and collection of single cells from within a heterogeneous population is required to produce genetically engineered cell lines, to develop new stem cell lines, and for single-cell studies. We describe a new platform for the positive selection of single live mammalian cells while the cells remain adherent to their growth surface. Cells were grown on arrays of microfabricated, releasable elements composed of SU-8 polymer termed "cell pallets". The presence of air between the elements restricted the cells to the top surfaces of the pallets. Single pallets situated within large arrays of pallets were released on demand using a single, focused, laser pulse. The laser pulses were low in energy (2-5 muJ) and did not detach nearby, nontargeted pallets. Since the SU-8 pallets and the underlying glass substrate were optically transparent, the cells on the pallets could be visualized by microscopy before and after release. Over 90% of cells remained attached to the pallet during laser-based release. The feasibility of growing the cells from the released pallets into clonal colonies was demonstrated. The pallet array system permits adherent cells to be inspected using conventional microscopy and selected cells released for further analysis. The ability to assess cells while they remain adherent to a surface will broaden the number of attributes that can be utilized for cell separation, for example, cell shape, cytoskeletal properties, and other attributes.
- Subjects :
- business.industry
Chemistry
Genetically engineered
Lasers
Positive selection
Nanotechnology
Cell Separation
Substrate (printing)
Laser
Analytical Chemistry
law.invention
Heterogeneous population
Tissue Array Analysis
law
On demand
Microscopy
Cell Adhesion
Animals
Optoelectronics
Stem cell line
business
Cells, Cultured
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....ca9851736542acae7d5d092cb8d59846