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Safety characterisation and inhibition of fungi and bacteria by a novel multiple enterocin-producing Enterococcus lactis 4CP3 strain
- Source :
- Microbial pathogenesis 118 (2018): 32–38. doi:10.1016/j.micpath.2018.03.005, info:cnr-pdr/source/autori:Ben Braiek O.; Cremonesi P.; Morandi S.; Smaoui S.; Hani K.; Ghrairi T./titolo:Safety characterisation and inhibition of fungi and bacteria by a novel multiple enterocin-producing Enterococcus lactis 4CP3 strain/doi:10.1016%2Fj.micpath.2018.03.005/rivista:Microbial pathogenesis/anno:2018/pagina_da:32/pagina_a:38/intervallo_pagine:32–38/volume:118
- Publication Year :
- 2018
- Publisher :
- Academic Press., London;, Regno Unito, 2018.
-
Abstract
- This study aims to characterise a potential bacteriocinogenic lactic acid bacterial strain isolated from a raw pink shrimp (Palaemon serratus) and evaluate its safety aspect. The strain designated as 4CP3 was noted to display antibacterial activities (P < 0.05) against Gram-positive and Gram-negative foodborne pathogens (Listeria monocytogenes and Pseudomonas aeruginosa) and some filamentous fungi (e.g. Aspergillus niger A79). Phenotypic and molecular techniques as well as phylogenetic analysis identified the isolate 4CP3 as Enterococcus lactis. Its produced antimicrobial substance was determined as a bacteriocin that was stable over a wide range of pH (2-10) and after heating at 100 °C for 15 min. The maximum bacteriocin production was 1400 AU/ml recorded after 12 h of incubation in de Man, Rogosa and Sharpe (MRS) broth medium at 30 °C. The mode of action of the bacteriocin produced by 4CP3 strain was identified as bactericidal against L. monocytogenes EGDe 107776 and P. aeruginosa ATCC 27853. By specific PCR amplifications, E. lactis 4CP3 was shown to produce the enterocins A, B and P. To our knowledge, this feature is newly described for E. lactis strain isolated from raw shrimps. Regarding safety aspect of E. lactis 4CP3, it has been demonstrated that this strain was not haemolytic, gelatinase negative, sensitive to vancomycin, and free of common antibiotic resistance genes and virulence factors. Therefore, it may be useful as a safe natural agent in preservation of foods or as a new probiotic strain in food and feed.
- Subjects :
- Bridged-Ring Compounds
0301 basic medicine
Hot Temperature
Virulence Factors
Enterocin
030106 microbiology
Antimicrobial activity
Microbiology
Foodborne Diseases
03 medical and health sciences
chemistry.chemical_compound
Bacteriocins
Species Specificity
Lactic acid bacteria
Animals
Phylogeny
Enterococcus lactis
Bacteria
biology
Strain (chemistry)
Probiotics
Fungi
Hydrogen-Ion Concentration
biology.organism_classification
Palaemon serratus
Bacterial strain
Anti-Bacterial Agents
Shrimp
Lactic acid
Infectious Diseases
chemistry
Palaemonidae
Safety
Shrimps
Enterococcus
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Microbial pathogenesis 118 (2018): 32–38. doi:10.1016/j.micpath.2018.03.005, info:cnr-pdr/source/autori:Ben Braiek O.; Cremonesi P.; Morandi S.; Smaoui S.; Hani K.; Ghrairi T./titolo:Safety characterisation and inhibition of fungi and bacteria by a novel multiple enterocin-producing Enterococcus lactis 4CP3 strain/doi:10.1016%2Fj.micpath.2018.03.005/rivista:Microbial pathogenesis/anno:2018/pagina_da:32/pagina_a:38/intervallo_pagine:32–38/volume:118
- Accession number :
- edsair.doi.dedup.....cad1c267a92fa59e3cb4fda7b012224f