Innovations in two-photon deep tissue microscopy

Authors :: Peter T. C. So
Ki Hean Kim
Christof Buehler
Chen-Yuan Dong
Barry R. Masters
Source :: IEEE Engineering in Medicine and Biology Magazine. 18:23-30
Publication Year :: 1999
Publisher :: Institute of Electrical and Electronics Engineers (IEEE), 1999.
Abstract: The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent spectroscopic measurements in deep tissue.