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Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men
- Source :
- Journal of Cellular Biochemistry. 119:10228-10238
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- At present, male contraceptive methods are only vasectomy and condoms, so it is necessary to research on male contraceptive techniques. The aim of this study is to observe the effects of scrotal heating (SH) on semen parameters, seminal l-carnitine (LC), epidermal growth factor (EGF), macrophage migration inhibitory factor (MIF), reproductive hormones and sperm chromosome numbers of adult healthy men, and to provide the experimental data for male contraception. The scrotums of 30 healthy male volunteers were exposed to the condition of 40 to 43°C SH belt warming 40 minutes each day for successive 2 days per week. The course of SH was continuous for 3 months. Computer-assisted semen analysis and hypo-osmotic swelling test, sperm DNA integrity, l-carnitine, MIF and EGF, and sperm fluorescence in situ hybridization were performed before, during, and after SH. The serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured by chemiluminescent immunoassay. The mean parameters of sperm concentration, vitality, and normal morphological sperm were significantly decreased in groups with sperms being collected during 1, 2, and 3 months of SH when compared with those in groups of pre-SH (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane functionality, levels of LC and MIF in semen, and LH, FSH, and T in serum were observed between the groups of before SH and after SH 3 months and the groups of during SH 1, 2, and 3 months (P < 0.001). The total rate of chromosome number for 13, 18, 21, X, and Y in the 3 months of SH was 13.7-fold greater (13.72%/1.69%) than before SH (P < 0.001). The constant SH can impact the semen quality, sperm DNA integrity, sperm chromosome, LC and MIF, and LH, FSH, and T in serum. Transient SH may be a new method for male contraception.
- Subjects :
- Adult
Male
0301 basic medicine
endocrine system
Semen
Male contraceptive
DNA Fragmentation
Semen analysis
Biology
Biochemistry
Heating
Andrology
03 medical and health sciences
Semen quality
Follicle-stimulating hormone
0302 clinical medicine
Carnitine
medicine
Humans
Testosterone
Macrophage Migration-Inhibitory Factors
Molecular Biology
In Situ Hybridization, Fluorescence
030219 obstetrics & reproductive medicine
Epidermal Growth Factor
Estradiol
medicine.diagnostic_test
urogenital system
Cell Biology
Luteinizing Hormone
Spermatozoa
Sperm
Intramolecular Oxidoreductases
Semen Analysis
030104 developmental biology
Scrotum
Follicle Stimulating Hormone
Luteinizing hormone
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....cb12cacc5acb5f7ae957768dbe75bb15