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Induced expression of c-fms in normal hematopoietic cells shows evidence for both conservation and lineage restriction of signal transduction in response to macrophage colony-stimulating factor
- Source :
- Blood. 83:972-981
- Publication Year :
- 1994
- Publisher :
- American Society of Hematology, 1994.
-
Abstract
- Retrovirus-mediated gene transfer was used to obtain expression of the macrophage colony-stimulating factor (MCSF) receptor, c-fms, on hematopoietic lineages that normally do not express this receptor. Cultures of murine fetal liver cells infected with the c-fms retrovirus developed erythroid colonies in cultures stimulated with M-CSF. However, these colonies were fewer and less hemoglobinized than colonies in parallel cultures stimulated by erythropoietin. Culture of isolated clones demonstrated a direct action of M-CSF on erythroid clones. Culture of c-fms retrovirus-infected adult murine bone marrow cells showed megakaryocyte and novel macrophage-megakaryocyte clones when stimulated by M-CSF. Culture of isolated clones again confirmed a direct action of M-CSF on megakaryocyte clones. In contrast, M-CSF stimulation of c-fms-infected granulocytes and granulocyte progenitor cells did not elicit proliferation, enhanced survival, or functional stimulation of granulocytes. These findings provide evidence for both conservation and lineage restriction of signal transduction in normal hematopoietic cells.
- Subjects :
- Genetic Vectors
Immunology
Gene Expression
Bone Marrow Cells
Receptor, Macrophage Colony-Stimulating Factor
Transfection
Biochemistry
Cell Line
Mice
Fetus
Granulocyte Colony-Stimulating Factor
Animals
Cells, Cultured
Macrophage Colony-Stimulating Factor
Cell Differentiation
Genes, fms
Cell Biology
Hematology
Flow Cytometry
Hematopoietic Stem Cells
Clone Cells
Mice, Inbred C57BL
Retroviridae
Liver
Mice, Inbred CBA
Signal Transduction
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....cb37d41cd4bf815a49360e9c7358f864