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Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke
- Source :
- Clinical science (London, England : 1979). 132(14)
- Publication Year :
- 2018
-
Abstract
- Long noncoding RNAs (lncRNAs) have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of the present study was to investigate the expression profile of lncRNAs in peripheral blood mononuclear cells (PBMCs) of acute IS patients and to explore their utility as biomarkers of IS. Distinctive expression patterns of PBMC lncRNAs were identified by an lncRNA microarray and individual quantitative real-time PCR (qRT-PCR) in four independent sets for 206 IS, 179 healthy controls (HCs), and 55 patients with transient ischemic attack (TIA). A biomarker panel (lncRNA-based combination index) was established using logistic regression. LncRNA microarray analysis showed 70 up-regulated and 128 down-regulated lncRNAs in IS patients. Individual qRT-PCR validation demonstrated that three lncRNAs (linc-DHFRL1-4, SNHG15, and linc-FAM98A-3) were significantly up-regulated in IS patients compared with HCs and TIA patients. Longitudinal analysis of lncRNA expression up to 90 days after IS showed that linc-FAM98A-3 normalized to control levels by day 7, while SNHG15 remained increased, indicating the ability of lncRNAs to monitor IS dynamics. Receiver-operating characteristic (ROC) curve analysis showed that the lncRNA-based combination index outperformed serum brain-derived neurotrophic factor (BDNF) and neurone-specific enolase (NSE) in distinguishing IS patients from TIA patients and HCs with areas under ROC curve of more than 0.84. Furthermore, the combination index increased significantly after treatment and was correlated with neurological deficit severity of IS. The panel of these altered lncRNAs was associated with acute IS and could serve as a novel diagnostic method.
- Subjects :
- 0301 basic medicine
Oncology
Male
medicine.medical_specialty
Microarray
Enolase
Peripheral blood mononuclear cell
Brain Ischemia
03 medical and health sciences
0302 clinical medicine
Neurotrophic factors
Internal medicine
medicine
Humans
Pathological
Cells, Cultured
Aged
Aged, 80 and over
Microarray analysis techniques
business.industry
Gene Expression Profiling
General Medicine
Middle Aged
Long non-coding RNA
Stroke
030104 developmental biology
Logistic Models
Case-Control Studies
Ischemic stroke
Leukocytes, Mononuclear
Female
RNA, Long Noncoding
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14708736
- Volume :
- 132
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- Clinical science (London, England : 1979)
- Accession number :
- edsair.doi.dedup.....cb4bdcbec51289a4a45f7497d9c138f1