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Comparison of commercial 5-aminolevulinic acid (Gliolan®) and the pharmacy-compounded solution fluorescence in glioblastoma

Authors :
Joan Bestard Escalas
Santiago Garfias Arjona
Marta Brell Doval
Priam Villalonga
Javier Pierola Lopetegui
Albert Maimó Barceló
Ruth Villalonga-Planells
Olga Delgado Sánchez
Javier Ángel Ibáñez Domínguez
Mónica Lara Almunia
Source :
Acta Neurochirurgica. 161:1733-1741
Publication Year :
2019
Publisher :
Springer Science and Business Media LLC, 2019.

Abstract

5-Aminolevulinic acid (5-ALA) has become an important assistant in glioblastoma (GB) surgery. Unfortunately, its price affects its widespread use. The aim of this study was to compare commercial 5-ALA with the pharmacy-compounded solution. Using first an in vitro experimental approach, different concentrations of the pharmacy-compounded solution and commercial 5-ALA were tested in U87MG, LN229, U373, and T98G commercial glioblastoma cell lines. Fluorescence intensity was compared for each concentration by flow cytometry. Mean fluorescence of culture supernatant and lysate samples were analyzed. In a second phase, both preparations were used for surgical glioblastoma resection and tumor samples were analyzed by confocal microscopy. Mean fluorescence intensity was analyzed for each preparation and compared. There was a high variability of fluorescence intensity between cell lines, but each cell line showed similar fluorescence for both preparations (compounded preparation and commercial 5-ALA). In the same way, both preparations had similar fluorescence intensity in glioblastoma samples. Both, compounded and commercial 5-ALA preparations produce equivalent fluorescent responses in human glioblastoma cells. Fluorescence intensity is cell line specific, but fluorescent properties of both preparations are undistinguishable.

Details

ISSN :
09420940 and 00016268
Volume :
161
Database :
OpenAIRE
Journal :
Acta Neurochirurgica
Accession number :
edsair.doi.dedup.....cb543fc205c4a5042fd73f7d6a443d80
Full Text :
https://doi.org/10.1007/s00701-019-03930-4