Back to Search
Start Over
Comparison of commercial 5-aminolevulinic acid (Gliolan®) and the pharmacy-compounded solution fluorescence in glioblastoma
- Source :
- Acta Neurochirurgica. 161:1733-1741
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- 5-Aminolevulinic acid (5-ALA) has become an important assistant in glioblastoma (GB) surgery. Unfortunately, its price affects its widespread use. The aim of this study was to compare commercial 5-ALA with the pharmacy-compounded solution. Using first an in vitro experimental approach, different concentrations of the pharmacy-compounded solution and commercial 5-ALA were tested in U87MG, LN229, U373, and T98G commercial glioblastoma cell lines. Fluorescence intensity was compared for each concentration by flow cytometry. Mean fluorescence of culture supernatant and lysate samples were analyzed. In a second phase, both preparations were used for surgical glioblastoma resection and tumor samples were analyzed by confocal microscopy. Mean fluorescence intensity was analyzed for each preparation and compared. There was a high variability of fluorescence intensity between cell lines, but each cell line showed similar fluorescence for both preparations (compounded preparation and commercial 5-ALA). In the same way, both preparations had similar fluorescence intensity in glioblastoma samples. Both, compounded and commercial 5-ALA preparations produce equivalent fluorescent responses in human glioblastoma cells. Fluorescence intensity is cell line specific, but fluorescent properties of both preparations are undistinguishable.
- Subjects :
- Lysis
030218 nuclear medicine & medical imaging
Flow cytometry
law.invention
03 medical and health sciences
0302 clinical medicine
Confocal microscopy
law
Cell Line, Tumor
medicine
Humans
Neurons
Photosensitizing Agents
Chromatography
medicine.diagnostic_test
Brain Neoplasms
business.industry
Mean fluorescence intensity
Aminolevulinic Acid
medicine.disease
Fluorescence
In vitro
Cell culture
Costs and Cost Analysis
Surgery
Neurology (clinical)
Glioblastoma
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 09420940 and 00016268
- Volume :
- 161
- Database :
- OpenAIRE
- Journal :
- Acta Neurochirurgica
- Accession number :
- edsair.doi.dedup.....cb543fc205c4a5042fd73f7d6a443d80
- Full Text :
- https://doi.org/10.1007/s00701-019-03930-4