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Protective effects of caffeic acid and caffeic acid phenethyl ester against acrolein-induced neurotoxicity in HT22 mouse hippocampal cells
- Source :
- Neuroscience Letters. 535:146-151
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Acrolein-induced oxidative stress is hypothesized to involve in the etiology of Alzheimer's disease (AD). Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) have antioxidative and neuroprotective properties. The present study investigated the protective effects of CA/CAPE on acrolein-induced oxidative neuronal toxicity. HT22 mouse hippocampal cells were pretreated with CA/CAPE and then exposed to acrolein. Cell viability, intracellular reactive oxygen species (ROS), and glutathione (GSH) level were measured. MAPKs and Akt/GSK3β signaling proteins as well as α/β-secretase of amyloid protein precursor were assayed by Western blotting. Pretreatment with CA/CAPE significantly attenuated acrolein-induced neurotoxicity, ROS accumulation, and GSH depletion. Further study suggested that CA/CAPE showed protective effects against acrolein by modulating MAPKs and Akt/GSK3β signaling pathways. Moreover, CA/CAPE restored the changes of β-secretase (BACE-1) and/or activation of α-secretase (ADAM-10) induced by acrolein. These findings suggest that CA/CAPE may provide a promising approach for the treatment of acrolein-related neurodegenerative diseases, such as AD.
- Subjects :
- Cell Survival
MAP Kinase Signaling System
education
Pharmacology
medicine.disease_cause
Hippocampus
Neuroprotection
Antioxidants
Cell Line
ADAM10 Protein
Glycogen Synthase Kinase 3
Mice
chemistry.chemical_compound
Caffeic Acids
medicine
Caffeic acid
Animals
Aspartic Acid Endopeptidases
Acrolein
Caffeic acid phenethyl ester
Protein kinase B
Neurons
Glycogen Synthase Kinase 3 beta
Chemistry
General Neuroscience
Neurotoxicity
Membrane Proteins
Glutathione
Phenylethyl Alcohol
medicine.disease
ADAM Proteins
Oxidative Stress
Neuroprotective Agents
Biochemistry
Amyloid Precursor Protein Secretases
Reactive Oxygen Species
Proto-Oncogene Proteins c-akt
geographic locations
Oxidative stress
Subjects
Details
- ISSN :
- 03043940
- Volume :
- 535
- Database :
- OpenAIRE
- Journal :
- Neuroscience Letters
- Accession number :
- edsair.doi.dedup.....cb5aa952264bce023fbcb7e14c91cf42