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Generation of single base-pair deletions, insertions, and substitutions by a site-specific recombination system
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 82(20)
- Publication Year :
- 1985
-
Abstract
- The sequence analysis of both products of individual phi 80 site-specific recombination events in vivo shows that recombination with a secondary attachment (att) site generates several different novel joints at the mismatched position: one recombination event resulted in a single base-pair deletion and two other recombination events resulted in two different single base-pair substitutions. The characterized products of recombination can be straightforwardly interpreted as the outcome of strand exchange involving staggered nicks bracketing the heterology within an overlap region of five to nine base pairs. In comparison, more complex segregation patterns have been observed in previous studies of lambda recombination between nonidentical att sites; the nature of the overlap region heterology may have a significant effect on the segregation patterns. To recover both products of a single recombination event, we used a plasmid that carries the phi 80 int and xis genes and both att sites. Because the two att sites are situated in opposite orientation, intramolecular recombination between them inverts rather than deletes the intervening segment of DNA. Although subsequent reinversion restores the original gross genetic arrangement, single base-pair insertions, deletions, and substitutions are introduced at the sites of recombination. One of the mutations improves the recombination efficiency of the secondary att site and thereby converts a formerly "stable" sequence to an efficient target for rearrangement, and other mutations are predicted to alter the specificity of recombination. These pathways may also provide useful models for the efficient generation of localized sequence diversity on a development (as well as evolutionary) time scale.
- Subjects :
- Genetics
Multidisciplinary
Base Sequence
Base pair
Genetic Vectors
Non-allelic homologous recombination
DNA, Recombinant
Cre recombinase
DNA Restriction Enzymes
Biology
Bacteriophage lambda
Sequence Homology, Nucleic Acid
Mutation
DNA Transposable Elements
Escherichia coli
Ectopic recombination
Cre-Lox recombination
Site-specific recombination
Chromosome Deletion
Cloning, Molecular
Floxing
Recombination
Plasmids
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 82
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....cb6c6f496e1dccf10e974f811a7827c4