Back to Search
Start Over
Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell line
- Source :
- Genome Research, 19(10), 1776-1785. Cold Spring Harbor Laboratory Press, Genome Research, 19(10), 1776-1785. COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
- Publication Year :
- 2009
-
Abstract
- Piwi proteins, a subclass of Argonaute-family proteins, carry ∼24–30-nt Piwi-interacting RNAs (piRNAs) that mediate gonadal defense against transposable elements (TEs). We analyzed the Drosophila ovary somatic sheet (OSS) cell line and found that it expresses miRNAs, endogenous small interfering RNAs (endo-siRNAs), and piRNAs in abundance. In contrast to intact gonads, which contain mixtures of germline and somatic cell types that express different Piwi-class proteins, OSS cells are a homogenous somatic cell population that expresses only PIWI and primary piRNAs. Detailed examination of its TE-derived piRNAs and endo-siRNAs revealed aspects of TE defense that do not rely upon ping-pong amplification. In particular, we provide evidence that a subset of piRNA master clusters, including flamenco, are specifically expressed in OSS and ovarian follicle cells. These data indicate that the restriction of certain TEs in somatic gonadal cells is largely mediated by a primary piRNA pathway.
- Subjects :
- Transposable element
endocrine system
Letter
Somatic cell
Molecular Sequence Data
Population
Piwi-interacting RNA
Biology
Models, Biological
Germline
Cell Line
Sequence Homology, Nucleic Acid
microRNA
RNA Precursors
Genetics
Animals
RasiRNA
RNA, Small Interfering
education
Genetics (clinical)
Regulation of gene expression
education.field_of_study
Base Sequence
urogenital system
Ovary
MicroRNAs
Gene Expression Regulation
Drosophila
Female
Subjects
Details
- Language :
- English
- ISSN :
- 10889051
- Volume :
- 19
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Genome Research
- Accession number :
- edsair.doi.dedup.....cb85f876b6f6dd3dbebb7dc6c6b87f3a