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Discovery and biochemical characterisation of four novel biomarkers for osteoarthritis
- Source :
- Annals of the rheumatic diseases. 70(6)
- Publication Year :
- 2011
-
Abstract
- ObjectiveKnee osteoarthritis (OA) is a heterogeneous, complex joint pathology of unknown aetiology. Biomarkers have been widely used to investigate OA but currently available biomarkers lack specificity and sensitivity. Therefore, novel biomarkers are needed to better understand the pathophysiological processes of OA initiation and progression.MethodsSurface enhanced laser desorption/ionisation-time of flight-mass spectrometry proteomic technique was used to analyse protein expression levels in 284 serum samples from patients with knee OA classified according to Kellgren and Lawrence (K&L) score (0–4). OA serum samples were also compared to serum samples provided by healthy individuals (negative control subjects; NC; n=36) and rheumatoid arthritis (RA) patients (n=25). Proteins that gave similar signal in all K&L groups of OA patients were ignored, whereas proteins with increased or decreased levels of expression were selected for further studies.ResultsTwo proteins were found to be expressed at higher levels in sera of OA patients at all four K&L scores compared to NC and RA, and were identified as V65 vitronectin fragment and C3fpeptide. Of the two remaining proteins, one showed increased expression (unknown protein at m/z of 3762) and the other (identified as connective tissue-activating peptide III protein) was decreased in K&L scores >2 subsets compared to NC, RA and K&L scores 0 or 1 subsets.ConclusionThe authors detected four unexpected biomarkers (V65 vitronectin fragment, C3f peptide, CTAP-III and m/z 3762 protein) that could be relevant in the pathophysiological process of OA as having significant correlation with parameters reflecting local inflammation and bone remodelling, as well as decrease in cartilage turnover.
- Subjects :
- Adult
Male
Proteomics
medicine.medical_specialty
Immunology
Osteoarthritis
General Biochemistry, Genetics and Molecular Biology
Arthritis, Rheumatoid
Diagnosis, Differential
Rheumatology
Internal medicine
Synovial Fluid
medicine
Immunology and Allergy
Humans
Aged
Aged, 80 and over
biology
business.industry
Cartilage
Case-control study
Reproducibility of Results
Blood Proteins
Middle Aged
Osteoarthritis, Knee
medicine.disease
Connective tissue disease
Pathophysiology
Peptide Fragments
medicine.anatomical_structure
Endocrinology
Rheumatoid arthritis
Case-Control Studies
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
biology.protein
Vitronectin
Female
business
Biomarkers
Subjects
Details
- ISSN :
- 14682060
- Volume :
- 70
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Annals of the rheumatic diseases
- Accession number :
- edsair.doi.dedup.....cb8d02165813fa69a4b977bf54857e8c