Back to Search
Start Over
Effect of Leukocyte Hydrolases on Bacteria. I. Degradation of 14C-Labeled Streptococcus and Staphylococcus by Leukocyte Lysates in Vitro
- Source :
- Journal of Infectious Diseases. 129:528-537
- Publication Year :
- 1974
- Publisher :
- Oxford University Press (OUP), 1974.
-
Abstract
- Lysates of human peripheral blood leukocytes that contained acid hydrolases solubilized 65% of the total radioactivity from 14C-labeled Staphylococcus albus after incubation for 18 hr at 37 C in acetate buffer, pH 5. Under similar conditions only 28%, 5.0%, and 2.5% of the total radioactivity was released from Staphylococcus aureus, group A Streptococcus, and A-variant streptococci, respectively. While the solubilization of radioactivity from S. albus was accompanied by the release of most of the glucosamine and by extensive cellular breakdown, the release of glucosamine and cellular breakdown of S. aureus were more moderate. However, no substantial degradation of streptococci was evident. Treatment with trypsin and lysozyme before addition of leukocyte lysates did not increase the solubilization and cellular breakdown of streptococci. However, such treatment greatly enhanced lysis of S. albus. Solubilization of radioactivity from staphylococci was accompanied by release of normal constituents of bacterial cell walls, e.g., glucosamine, muramic acid, alanine, lysine, glutamic acid, and ribitol (as revealed by paper chromatography). Under similar conditions, only alanine and a small portion of glucosamine were released from streptococci after treatment with leukocyte lysates. The use of radiochromatography and electron microscopy for study of the interaction of leukocyte enzymes with bacteria may contribute to our understanding of the interrelationship of leukocytes and bacteria.
- Subjects :
- Lysis
Chromatography, Paper
Staphylococcus
Acid Phosphatase
Muramic acid
medicine.disease_cause
Bacterial cell structure
Microbiology
chemistry.chemical_compound
Phenols
Cell Wall
Glucosamine
Leukocytes
medicine
Humans
Immunology and Allergy
Trypsin
Carbon Radioisotopes
Glucuronidase
biology
Tissue Extracts
Streptococcus
Hydrogen-Ion Concentration
biology.organism_classification
Cathepsins
Teichoic Acids
Microscopy, Electron
Infectious Diseases
Biochemistry
chemistry
Streptococcus pyogenes
Muramidase
Lysozyme
Bacteria
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 129
- Database :
- OpenAIRE
- Journal :
- Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....cba0f1bf185e7815b57d269ac084b767