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Binding of guide piRNA triggers methylation of the unstructured N-terminal region of Aub leading to assembly of the piRNA amplification complex
- Source :
- Nature Communications, Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
- Publication Year :
- 2021
- Publisher :
- Nature Publishing Group, 2021.
-
Abstract
- PIWI proteins use guide piRNAs to repress selfish genomic elements, protecting the genomic integrity of gametes and ensuring the fertility of animal species. Efficient transposon repression depends on amplification of piRNA guides in the ping-pong cycle, which in Drosophila entails tight cooperation between two PIWI proteins, Aub and Ago3. Here we show that post-translational modification, symmetric dimethylarginine (sDMA), of Aub is essential for piRNA biogenesis, transposon silencing and fertility. Methylation is triggered by loading of a piRNA guide into Aub, which exposes its unstructured N-terminal region to the PRMT5 methylosome complex. Thus, sDMA modification is a signal that Aub is loaded with piRNA guide. Amplification of piRNA in the ping-pong cycle requires assembly of a tertiary complex scaffolded by Krimper, which simultaneously binds the N-terminal regions of Aub and Ago3. To promote generation of new piRNA, Krimper uses its two Tudor domains to bind Aub and Ago3 in opposite modification and piRNA-loading states. Our results reveal that post-translational modifications in unstructured regions of PIWI proteins and their binding by Tudor domains that are capable of discriminating between modification states is essential for piRNA biogenesis and silencing.<br />PIWI protein contains arginine rich motifs that are post-translationally modified to symmetrically methylated arginine (sDMA) residues. Here the authors show that piRNA loading into Aub triggers sDMA modification which is recognized by Krimper to promote formation of Krimper-Aub-Ago3 complex for piRNA amplification in Drosophila.
- Subjects :
- Male
Models, Molecular
0301 basic medicine
Transposable element
Protein-Arginine N-Methyltransferases
endocrine system
Science
Protein domain
General Physics and Astronomy
Piwi-interacting RNA
Biology
Methylation
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Protein Domains
Peptide Initiation Factors
Gene silencing
Animals
Drosophila Proteins
RNA, Small Interfering
Psychological repression
X-ray crystallography
Methylosome
Multidisciplinary
urogenital system
Protein arginine methyltransferase 5
General Chemistry
Cell biology
030104 developmental biology
Argonaute Proteins
Piwi RNAs
Drosophila
Female
Carrier Proteins
Protein Processing, Post-Translational
030217 neurology & neurosurgery
Biogenesis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
- Accession number :
- edsair.doi.dedup.....cbda6d45785e03170dfb0c00cdfa12b6