Back to Search
Start Over
Perturbation of Serotonin Homeostasis during Adulthood Affects Serotonergic Neuronal Circuitry
- Source :
- eNeuro
- Publication Year :
- 2017
-
Abstract
- Visual Abstract<br />Growing evidence shows that the neurotransmitter serotonin (5-HT) modulates the fine-tuning of neuron development and the establishment of wiring patterns in the brain. However, whether serotonin is involved in the maintenance of neuronal circuitry in the adult brain remains elusive. Here, we use a Tph2fl°x conditional knockout (cKO) mouse line to assess the impact of serotonin depletion during adulthood on serotonergic system organization. Data show that the density of serotonergic fibers is increased in the hippocampus and decreased in the thalamic paraventricular nucleus (PVN) as a consequence of brain serotonin depletion. Strikingly, these defects are rescued following reestablishment of brain 5-HT signaling via administration of the serotonin precursor 5-hydroxytryptophan (5-HTP). Finally, 3D reconstruction of serotonergic fibers reveals that changes in serotonin homeostasis affect axonal branching complexity. These data demonstrate that maintaining proper serotonin homeostasis in the adult brain is crucial to preserve the correct serotonergic axonal wiring.
- Subjects :
- Messenger
Tryptophan Hydroxylase
Inbred C57BL
Transgenic
Serotonergic innervation
5-Hydroxytryptophan
chemistry.chemical_compound
Mice
0302 clinical medicine
Serotonin Agents
Neural Pathways
Homeostasis
Neurotransmitter
Serotonin Plasma Membrane Transport Proteins
0303 health sciences
Serotonergic Neuron
5-hydroxytryptophan
serotonergic innervations
serotonin
serotonin homeostasis
tryptophan hydroxylase 2
Animals
Brain-Derived Neurotrophic Factor
Gene Expression Regulation
Green Fluorescent Proteins
Mice, Inbred C57BL
Mice, Transgenic
Midline Thalamic Nuclei
Nerve Net
RNA, Messenger
Serotonergic Neurons
Serotonin
Signal Transduction
General Neuroscience
Medicine (all)
General Medicine
New Research
Serotonin homeostasi
medicine.anatomical_structure
Serotonin Agent
Serotonin Plasma Membrane Transport Protein
Biology
Development
Serotonergic
Green Fluorescent Protein
03 medical and health sciences
Neural Pathway
Homeostasi
medicine
030304 developmental biology
Brain-derived neurotrophic factor
Animal
chemistry
RNA
Neuron
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- eNeuro
- Accession number :
- edsair.doi.dedup.....cc06fb3efdd8b3d68aedfb6d5bd7f546