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Forsythoside A Inhibits BVDV Replication via TRAF2-Dependent CD28–4-1BB Signaling in Bovine PBMCs
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 9, p e0162791 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Bovine viral diarrhea virus (BVDV), the causative agent of bovine viral diarrhea/mucosal disease (BVD/MD), is an important pathogen of cattle and other wild animals throughout the world. BVDV infection typically leads to an impaired immune response in cattle. In the present study, we investigated the effect of Forsythoside A (FTA) on BVDV infection of bovine peripheral blood mononuclear cells (PBMCs). We found that Forsythoside A could not only promote proliferation of PBMCs and T cells activation but also inhibit the replication of BVDV as well as apoptosis induced by BVDV. FTA treatment could counteract the BVDV-induced overproduction of IFN-γ to maintain the immune homeostasis in bovine PBMCs. At same time, FTA can enhance the secretion of IL-2. What's more, BVDV promotes the expression of CD28, 4-1BB and TRAF-2, which can be modulated by FTA. Our data suggest that FTA protects PBMCs from BVDV infection possibly via TRAF2-dependent CD28-4-1BB signaling, which may activate PBMCs in response to BVDV infection. Therefore, this aids in the development of an effective adjuvant for vaccines against BVDV and other specific FTA-based therapies for preventing BVDV infection.
- Subjects :
- 0301 basic medicine
animal diseases
viruses
Protein Expression
lcsh:Medicine
Apoptosis
Lymphocyte Activation
Virus Replication
Immune Receptors
Biochemistry
Monocytes
Immunoglobulin G
White Blood Cells
Animal Cells
Medicine and Health Sciences
Cytotoxic T cell
Glycosides
lcsh:Science
Immune Response
Pathogen
Cells, Cultured
Mammals
Immune System Proteins
Multidisciplinary
Cell Death
T Cells
virus diseases
Viral Load
Cell Processes
Vertebrates
Cytokines
Female
Cellular Types
Viral load
Signal Transduction
Research Article
Immune Cells
Immunology
Cytotoxic T cells
Biology
Research and Analysis Methods
Microbiology
complex mixtures
Peripheral blood mononuclear cell
Virus
03 medical and health sciences
Immune system
CD28 Antigens
Bovines
Virology
Gene Expression and Vector Techniques
Animals
Molecular Biology Techniques
Molecular Biology
Molecular Biology Assays and Analysis Techniques
Diarrhea Viruses, Bovine Viral
Blood Cells
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Cell Biology
biochemical phenomena, metabolism, and nutrition
TNF Receptor-Associated Factor 2
Viral Replication
T Cell Receptors
030104 developmental biology
Viral replication
Amniotes
biology.protein
lcsh:Q
Cattle
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....cc083da302e5ad52066254f41dc040f7