Fluoroquinolone photodegradation influences specific basophil activation

Fluoroquinolones (FQs) are photoreactive drugs, but it is not known whether laboratory light exposure can influence the induction of photoproducts and modify in vitro test results. The basophil activation test (BAT) has proven to be useful for evaluating immunoglobulin E (IgE)-mediated hypersensitivity to FQs, with a higher percentage of positive responders with ciprofloxacin (CIP) than with moxifloxacin (MOX). We studied the effect of laboratory light on CIP and MOX degradation, and drug-protein conjugate formation, and its influence on the BAT for evaluating IgE-mediated hypersensitivity to FQs. The results showed an important decrease in fluorescence emission intensity under light compared to dark conditions for MOX, and that BAT positivity was lower in light (17.9%) than in dark (35.7%) conditions. No changes were found for CIP in either fluorescence emission intensity or BAT results (46.4% in both conditions). We can conclude that light exposure is a critical factor in BAT results when photolabile drugs like MOX are used. Therefore, light is important when interpreting in vitro results. Copyright (C) 2012 S. Karger AG, Basel
We thank Ian Johnstone for his help with the English language version of the manuscript, and Jose Luis Rodriguez-Bada and Lidia Melendez for their technical support. This study was funded by the FIS-Thematic Networks and Co-operative Research Centres: RIRAAF (RD07/0064) and the Spanish Health Ministry (FIS PS09/01768, PI11/01416 and M Servet contract CP11/00154 for I.A.), the Andalusia Health Ministry (PI-0545-2010), and the Andalusia Economic Innovation and Science Ministry (CTS 06603).