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Combined effects of high hydrostatic pressure and dispersed oil on the metabolism and the mortality of turbot hepatocytes (Scophthalmus maximus)
- Source :
- Chemosphere, Chemosphere, Elsevier, 2020, 249, pp.126420-. ⟨10.1016/j.chemosphere.2020.126420⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- Since the DeepWater Horizon oil spill and the use at 1450 m depth of dispersant as a technical response, the need of relevant ecotoxicological data on deep-sea ecosystems becomes crucial. In this context, this study focused on the effect of high hydrostatic pressure (10.1 MPa) on turbot hepatocytes isolated from fish exposed either to chemically dispersed oil, mechanically dispersed oil or dispersant alone. Potential combined effects of oil/dispersant and hydrostatic pressure, were assessed on cell mortality (total cell death, necrosis and apoptosis), cell viability and on hepatocyte oxygen consumption (MO2). No change in cell mortality was observed in any of the experimental conditions, whereas, the results of cell viability showed a strong and significant increase in the two oil groups independently of the pressure exposure. Finally, oil exposure and hydrostatic pressure have additive effects on oxygen consumption at a cellular level. Presence of dispersant prevent any MO2 increase in our experimental conditions. These mechanistic effects leading to this increased energetic demand and its eventual inhibition by dispersant must be investigated in further experiments.
- Subjects :
- Environmental Engineering
Health, Toxicology and Mutagenesis
0208 environmental biotechnology
Hydrostatic pressure
chemistry.chemical_element
Context (language use)
02 engineering and technology
010501 environmental sciences
Ecotoxicology
01 natural sciences
Oxygen
Dispersant
Hydrostatic Pressure
Animals
Environmental Chemistry
Petroleum Pollution
14. Life underwater
Viability assay
Food science
Ecosystem
0105 earth and related environmental sciences
biology
Public Health, Environmental and Occupational Health
General Medicine
General Chemistry
Metabolism
biology.organism_classification
Pollution
020801 environmental engineering
Scophthalmus
Turbot
Petroleum
Seafood
chemistry
[SDE]Environmental Sciences
Flatfishes
Hepatocytes
Water Pollutants, Chemical
Subjects
Details
- Language :
- English
- ISSN :
- 00456535
- Database :
- OpenAIRE
- Journal :
- Chemosphere, Chemosphere, Elsevier, 2020, 249, pp.126420-. ⟨10.1016/j.chemosphere.2020.126420⟩
- Accession number :
- edsair.doi.dedup.....cc2ad82dbca5fba11955a432f220ef14