Back to Search
Start Over
Effects of tremella–alginate–liposome encapsulation on oral delivery of inactivated H5N3 vaccine
- Source :
- Journal of Microencapsulation. 28:55-61
- Publication Year :
- 2010
- Publisher :
- Informa UK Limited, 2010.
-
Abstract
- In this study, we evaluated a system for oral vaccine delivery, consisting of liposomes coated first with a layer of tremella and then with an outer layer of acid-induced alginate. In vitro release studies showed that the triple layer of alginate-tremella-liposomes was more resistant to an acidic pH and modulated the release profiles at an alkaline pH. Transepithelial electrical resistance (TEER) studies revealed that liposomes or tremella-coated liposomes were able to open tight junctions of the Caco-2 cell monolayer. In mice, although serum immunoglobulin G (IgG) was not expected to increase and haemagglutination inhibition showed that antibody levels were still too low to provide sufficient protection, alginate-tremella-liposomes encapsulated virus-induced intestinal secretory immunoglobulin A (s-IgA) production to provide protection against virus infection. In conclusion, an oral virus vaccine entrapped in alginate-tremella-liposomes improved the mucosal antiviral s-IgA response. This system may have potential use as a carrier for oral vaccine delivery.
- Subjects :
- Immunoglobulin A
Alginates
Administration, Oral
Pharmaceutical Science
Bioengineering
Tremella
Immunoglobulin G
Microbiology
Mice
Colloid and Surface Chemistry
Glucuronic Acid
Orthomyxoviridae Infections
Antigen
Animals
Humans
Physical and Theoretical Chemistry
Antigens, Viral
Mice, Inbred BALB C
Liposome
biology
Tight junction
Hexuronic Acids
Organic Chemistry
Hemagglutination Inhibition Tests
biology.organism_classification
In vitro
Vaccines, Inactivated
Influenza A virus
Influenza Vaccines
Caco-2
Antibody Formation
Immunoglobulin A, Secretory
Liposomes
biology.protein
Female
Caco-2 Cells
Subjects
Details
- ISSN :
- 14645246 and 02652048
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Microencapsulation
- Accession number :
- edsair.doi.dedup.....cc45ad1a89dd343c77e6c5939ad5bd9c