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Genetic and genomic diversity of NheABC locus from Bacillus strains
- Source :
- Archives of Microbiology
- Publisher :
- Springer Nature
-
Abstract
- Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurrence of NHE among them, and other members of Bacillus is unclear. Consequently, we aimed to determine the distribution and evolution of NHE within Bacillus by confirming the presence of the nheA, B and C sequences and variation within them using published data, and to analyze the genomic and genetic diversity. The phylogenetic tree of NHE proteins (NheA, NheB and NheC) from 81 different B. cereus s.l. strains was constructed. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis. Analysis of the genomic diversity of the nheA, B and C loci revealed that their upstream regions were more conserved than the downstream sequences. Multilocus sequence typing schemes (MLST) based on seven concatenated housekeeping genes and nheA, B and C genes of the 75 strains were developed. The neighbor joining phylogenetic tree based on seven housekeeping genes together with nheA, B and C genes was similiar to published Bacillus phylogenetic trees. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis.The results indicate that nheA, B and C genes do not affect the diversity of housekeeping genes, and this specific NHE protein does not participate in the diarrheic syndrome. Electronic supplementary material The online version of this article (doi:10.1007/s00203-017-1350-9) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Virulence Factors
030106 microbiology
Bacterial Toxins
Bacillus cereus
Virulence
Locus (genetics)
Bacillus
Biochemistry
Microbiology
Genetic diversity
Foodborne Diseases
03 medical and health sciences
Enterotoxins
Bacterial Proteins
Genetics
Humans
Gene
Molecular Biology
Phylogeny
Original Paper
biology
Phylogenetic tree
General Medicine
biology.organism_classification
Housekeeping gene
030104 developmental biology
Cereus
Multilocus sequence typing
NheABC
MLST
Multilocus Sequence Typing
Subjects
Details
- Language :
- English
- ISSN :
- 03028933
- Database :
- OpenAIRE
- Journal :
- Archives of Microbiology
- Accession number :
- edsair.doi.dedup.....cc52f23607b849c7db4ba583bfc3777f
- Full Text :
- https://doi.org/10.1007/s00203-017-1350-9