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Visualization and quantification of pancreatic tumor stroma in fresh tissue via ultraviolet surface excitation

Authors :
Tayyaba Hasan
P. Jack Hoopes
Phuong Vincent
Scott M. Palisoul
Jason R. Gunn
Kimberley S. Samkoe
Brian W. Pogue
Petr Bruza
Source :
Journal of Biomedical Optics
Publication Year :
2021
Publisher :
SPIE-Intl Soc Optical Eng, 2021.

Abstract

Significance: The study has confirmed the feasibility of using ultraviolet (UV) excitation to visualize and quantify desmoplasia in fresh tumor tissue of pancreatic adenocarcinoma (PDAC) in an orthotopic xenograft mouse model, which provides a useful imaging platform to evaluate acute therapeutic responses. Aim: Stromal network of collagen prominent in PDAC tumors is examined by imaging fresh tissue samples stained with histological dyes. Fluorescence signals are color-transferred to mimic Masson’s trichrome staining. Approach: Murine tumor samples were stained with Hoechst, eosin, and rhodamine B and excited at 275-nm. Fluorescence signals in the visible spectrum were captured by a CMOS color camera with high contrast and resolution at whole-tumor slice field of view. Results: Fluorescence imaging using UV excitation is capable of visualizing collagen deposition in PDAC tumors. Both fluorescence and histology data showed collagen content of up to 30%. The collagen modulation effect due to photodynamic priming treatment was observed showing 13% of collagen reduction. Necrosis area is visible and perfusion imaging using Texas Red dextran is feasible. Conclusions: The study demonstrates collagen visualization in fresh PDAC tumor samples using UV excitation. This imaging platform also provides quantitative stromal information from fiber analysis and visibility of necrosis and perfusion, suitable for therapeutic response assessment of photodynamic therapy.

Details

ISSN :
10833668
Volume :
26
Database :
OpenAIRE
Journal :
Journal of Biomedical Optics
Accession number :
edsair.doi.dedup.....cc5cb7f6c185c67666d3e6bdb8bfdb6d
Full Text :
https://doi.org/10.1117/1.jbo.26.1.016002