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Circulating miRNA in Patients Undergoing Total Pancreatectomy and Islet Autotransplantation
- Source :
- Cell Transplantation, Cell Transplantation, Vol 30 (2021)
- Publication Year :
- 2021
- Publisher :
- SAGE Publications, 2021.
-
Abstract
- Circulating microRNAs (miRNAs) can be biomarkers for diagnosis and progression of several pathophysiological conditions. In a cohort undergoing total pancreatectomy with islet autotransplantation (TPIAT) from the multicenter Prospective Observational Study of TPIAT (POST), we investigated associations between a panel of circulating miRNAs (hsa-miR-375, hsa-miR-29b-3p, hsa-miR-148a-3p, hsa-miR-216a-5p, hsa-miR-320d, hsa-miR-200c, hsa-miR-125b, hsa-miR-7-5p, hsa-miR-221-3p, hsa-miR-122-5p) and patient, disease and islet-isolation characteristics. Plasma samples ( n = 139) were collected before TPIAT and miRNA levels were measured by RTPCR. Disease duration, prior surgery, and pre-surgical diabetes were not associated with circulating miRNAs. Levels of hsa-miR-29b-3p ( P = 0.03), hsa-miR-148a-3p ( P = 0.04) and hsa-miR-221-3p ( P = 0.01) were lower in those with genetic risk factors. Levels of hsa-miR-148a-3p ( P = 0.04) and hsa-miR-7-5p ( P = 0.04) were elevated in toxic/metabolic disease. Participants with exocrine insufficiency had lower hsa-miR-29b-3p, hsa-miR-148a-3p, hsa-miR-320d, hsa-miR-221-3p ( P < 0.01) and hsa-miR-375, hsa-miR-200c-3p, and hsa-miR-125b-5p ( P < 0.05). Four miRNAs were associated with fasting C-peptide before TPIAT (hsa-miR-29b-3p, r = 0.18; hsa-miR-148a-3p, r = 0.21; hsa-miR-320d, r = 0.19; and hsa-miR-221-3p, r = 0.21; all P < 0.05), while hsa-miR-29b-3p was inversely associated with post-isolation islet equivalents/kg and islet number/kg ( r = −0.20, P = 0.02). Also, hsa-miR-200c ( r = 0.18, P = 0.03) and hsa-miR-221-3p ( r = 0.19, P = 0.03) were associated with islet graft tissue volume. Further investigation is needed to determine the predictive potential of these miRNAs for assessing islet autotransplant outcomes.
- Subjects :
- Adult
Male
medicine.medical_specialty
medicine.medical_treatment
Biomedical Engineering
Islets of Langerhans Transplantation
Gastroenterology
Transplantation, Autologous
chronic pancreatitis
03 medical and health sciences
Islets of Langerhans
0302 clinical medicine
Pancreatectomy
Internal medicine
Diabetes mellitus
medicine
circulating miRNAs
Humans
Prospective Studies
Transplantation
geography
geography.geographical_feature_category
business.industry
islet transplantation
Cell Biology
medicine.disease
Islet
Pathophysiology
Autotransplantation
body regions
Circulating MicroRNA
MicroRNAs
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Cohort
embryonic structures
Biomarker (medicine)
Medicine
biomarker
030211 gastroenterology & hepatology
Original Article
Female
business
Subjects
Details
- Language :
- English
- ISSN :
- 15553892 and 09636897
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Cell Transplantation
- Accession number :
- edsair.doi.dedup.....cc61f42e2906f96b8f3632f48731c410