Back to Search
Start Over
Borrelia burgdorferi Sensu Stricto Is Clonal in Patients with Early Lyme Borreliosis ▿
- Publication Year :
- 2008
- Publisher :
- American Society for Microbiology (ASM), 2008.
-
Abstract
- Lyme borreliosis, the most commonly reported vector-borne disease in North America, is caused by the spirochete Borrelia burgdorferi . Given the extensive genetic polymorphism of B . burgdorferi , elucidation of the population genetic structure of the bacterium in clinical samples may be relevant for understanding disease pathogenesis and may have applicability for the development of diagnostic tests and vaccine preparations. In this investigation, the genetic polymorphism of the 16S-23S rRNA ( rrs - rrlA ) intergenic spacer and ospC was investigated at the sequence level in 127 clinical isolates obtained from patients with early Lyme borreliosis evaluated in suburban New York City. Sixteen distinct rrs - rrlA and 16 distinct ospC alleles were identified, representing virtually all of the genotypes previously found in questing Ixodes scapularis nymphs in this region. In addition, a new ospC group was identified in a single patient. The strong linkage observed between the chromosome-located rrs - rrlA and plasmid-borne ospC genes suggests a clonal structure of B . burgdorferi in these isolates, despite evidence of recombination at ospC .
- Subjects :
- DNA, Bacterial
Population
Molecular Sequence Data
Spirochaetaceae
Public Health Microbiology
Applied Microbiology and Biotechnology
Lyme disease
Genotype
medicine
Humans
Borrelia burgdorferi
Allele
education
Phylogeny
education.field_of_study
Antigens, Bacterial
Likelihood Functions
Lyme Disease
Polymorphism, Genetic
Ecology
biology
Base Sequence
biology.organism_classification
medicine.disease
bacterial infections and mycoses
Virology
Bacterial Typing Techniques
Ixodes scapularis
Genetic structure
DNA, Intergenic
New York City
Sequence Alignment
Food Science
Biotechnology
Bacterial Outer Membrane Proteins
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....cc72479c8a8ac5120e2d2959e72ff51a