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Absence of Testicular Estrogen Leads to Defects in Spermatogenesis and Increased Semen Abnormalities in Male Rabbits

Authors :
Aurélie Dewaele
Emilie Dujardin
Marjolaine André
Audrey Albina
Hélène Jammes
Frank Giton
Eli Sellem
Geneviève Jolivet
Eric Pailhoux
Maëlle Pannetier
Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED)
École nationale vétérinaire - Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Institut Mondor de Recherche Biomédicale (IMRB)
Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Hôpital Henri Mondor
ANR-09-GENM-0009,GENIDOV,Réseaux géniques impliqués dans la différenciation ovarienne chez la chèvre et la souris(2009)
ANR-13-BSV2-0017,ARGONADS,Acide rétinoique et devenir des cellules germinales(2013)
ANR-20-CE14-0022,ARDIGERM,Acide rétinoïque dans la différenciation des cellules germinales et la méiose(2020)
BREED, INRAE
Génomique et biotechnologies végétales - Réseaux géniques impliqués dans la différenciation ovarienne chez la chèvre et la souris - - GENIDOV2009 - ANR-09-GENM-0009 - GENOM-BTV - VALID
Blanc 2013 - Acide rétinoique et devenir des cellules germinales - - ARGONADS2013 - ANR-13-BSV2-0017 - Blanc 2013 - VALID
Acide rétinoïque dans la différenciation des cellules germinales et la méiose - - ARDIGERM2020 - ANR-20-CE14-0022 - AAPG2020 - VALID
Source :
Genes, Genes, 2022, 13, ⟨10.3390/genes13112070⟩, Genes; Volume 13; Issue 11; Pages: 2070
Publication Year :
2022
Publisher :
HAL CCSD, 2022.

Abstract

Estrogens are steroid hormones produced by the aromatization of androgens by the aromatase enzyme, encoded by the CYP19A1 gene. Although generally referred to as “female sex hormones”, estrogen is also produced in the adult testes of many mammals, including humans. To better understand the function of estrogens in the male, we used the rabbit model which is an important biomedical model. First, the expression of CYP19A1 transcripts was localized mainly in meiotic germ cells. Thus, testicular estrogen appears to be produced inside the seminiferous tubules. Next, the cells expressing ESR1 and ESR2 were identified, showing that estrogens could exert their function on post-meiotic germ cells in the tubules and play a role during sperm maturation, since ESR1 and ESR2 were detected in the cauda epididymis. Then, CRISPR/Cas9 CYP19A1−/− genetically modified rabbits were analyzed. CYP19A1−/− males showed decreased fertility with lower sperm count associated with hypo-spermatogenesis and lower spermatid number. Germ/sperm cell DNA methylation was unchanged, while sperm parameters were affected as CYP19A1−/− males exhibited reduced sperm motility associated with increased flagellar defects. In conclusion, testicular estrogens could be involved in the spermatocyte–spermatid transition in the testis, and in the acquisition of sperm motility in the epididymis.

Details

Language :
English
ISSN :
20734425
Database :
OpenAIRE
Journal :
Genes, Genes, 2022, 13, ⟨10.3390/genes13112070⟩, Genes; Volume 13; Issue 11; Pages: 2070
Accession number :
edsair.doi.dedup.....cc7a600fee1de8ffbf201b0d443a1ae7
Full Text :
https://doi.org/10.3390/genes13112070⟩