Back to Search
Start Over
Fusaristatin A production negatively affects the growth and aggressiveness of the wheat pathogen Fusarium pseudograminearum
Fusaristatin A production negatively affects the growth and aggressiveness of the wheat pathogen Fusarium pseudograminearum
- Source :
- Khudhair, M, Kazan, K, Thatcher, L, Obanor, F, Rusu, A, Sørensen, J L, Wollenberg, R D, McKay, A, Giblot-Ducray, D, Simpfendorfer, S, Aitken, E & Gardiner, D M 2020, ' Fusaristatin A production negatively affects the growth and aggressiveness of the wheat pathogen Fusarium pseudograminearum ', Fungal Genetics and Biology, vol. 136, 103314 . https://doi.org/10.1016/j.fgb.2019.103314
- Publication Year :
- 2019
-
Abstract
- Fusarium pseudograminearum (Fp), the causative fungal pathogen of the diseases Fusarium crown rot, is an important constraint to cereals production in many countries including Australia. Fp produces a number of secondary metabolites throughout its life cycle. One of these metabolites, the cyclic lipopeptide fusaristatin A, is encoded by a specific gene cluster containing a polyketide synthase and a three-module non-ribosomal peptide synthetase. However, a recent survey of Fp populations across Australia suggests that this cluster may only be present in a subset of isolates from Western Australia (WA). In this study, we screened 319 Fp isolates from WA and 110 Fp isolates from the Australian eastern states of New South Wales, Victoria, Queensland and South Australia to examine the distribution of this gene cluster among Australian Fp populations. The fusaristatin A gene cluster was found to be present in ~50% of Fp isolates from WA but completely absent in Fp isolates from eastern states. To determine its potential function, mutants of the fusaristatin A gene cluster were generated by disrupting the non-ribosomal peptide synthetase and polyketide synthase genes simultaneously in two different parental backgrounds. The mutants showed increased growth rates and were significantly more aggressive than their respective parental strains on wheat in crown rot pathogenicity assays. This suggested that fusaristatin A has a negative effect on fungal development and aggressiveness. The possible reasons for the geographically restricted presence of the fusaristatin A gene cluster and its role in fungal biology are discussed.
- Subjects :
- Fusarium
Fusarium pseudograminearum
Mutant
Fusarium crown rot
Disease cluster
Polyketide
Microbiology
Fungal Proteins
03 medical and health sciences
Gene Knockout Techniques
Aggressiveness
Polyketide synthase
Depsipeptides
Gene cluster
Genetics
Peptide Synthases
DNA, Fungal
Pathogen
Gene
Triticum
030304 developmental biology
Plant Diseases
Head blight
0303 health sciences
biology
Host Microbial Interactions
030306 microbiology
Secondary metabolites
Australia
biology.organism_classification
fusaristatin A gene cluster
Multigene Family
biology.protein
Edible Grain
Polyketide Synthases
Subjects
Details
- ISSN :
- 10960937
- Volume :
- 136
- Database :
- OpenAIRE
- Journal :
- Fungal genetics and biology : FGB
- Accession number :
- edsair.doi.dedup.....cc85a44a86bafac7b3b50ea7264d6379
- Full Text :
- https://doi.org/10.1016/j.fgb.2019.103314