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The adipokine apelin-13 induces expression of prothrombotic tissue factor
- Source :
- Thrombosis and haemostasis 113 (2015): 363–372. doi:10.1160/TH14-05-0451, info:cnr-pdr/source/autori:Cirillo P.; Ziviello F.; Pellegrino G.; Conte S.; Cimmino G.; Giaquinto A.; Pacifico F.; Leonardi A.; Golino P.; Trimarco B./titolo:The adipokine apelin-13 induces expression of prothrombotic tissue factor/doi:10.1160%2FTH14-05-0451/rivista:Thrombosis and haemostasis/anno:2015/pagina_da:363/pagina_a:372/intervallo_pagine:363–372/volume:113, Thrombosis and haemostasis (2014)., info:cnr-pdr/source/autori:Cirillo P1, Ziviello F, Pellegrino G, Conte S, Cimmino G, Giaquinto A, Pacifico F, Leonardi A, Golino P, Trimarco B./titolo:The adipokine apelin-13 induces expression of prothrombotic tissue factor./doi:/rivista:Thrombosis and haemostasis/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Publication Year :
- 2015
- Publisher :
- Schattauer., Stuttgart , Germania, 2015.
-
Abstract
- SummaryAdipocytes are cells able to produce and secrete several active substances (adipokines) with direct effects on vascular cells. Apelin, one of the most recently identified adipokines has been studied in cardiovascular system physiology in regard to vessel vasodilation and myocardial contraction, but it has not yet completely characterised for its pathophysiological role in cardiovascular disease and especially in acute coronary syndromes (ACS). Several studies have indicated that tissue factor (TF) plays a pivotal role in the pathophysiology of ACS by triggering the formation of intracoronary thrombi following endothelial injury. This study investigates the effects of apelin 12 and apelin 13 on TF in human umbilical endothelial cells (HUVECs) and monocytes. Cells were stimulated with increasing concentrations of apelin 12 or apelin 13 and then processed to evaluate TF-mRNA levels by real-time PCR as well as TF expression/activity by FACS analysis and pro-coagulant activity. Finally, a potential molecular pathway involved in modulating this phenomenon was investigated. We demonstrate that apelin 13 but not apelin 12 induces transcription of mRNA for TF. In addition, we show that this adipokine promotes surface expression of TF that is functionally active. Apelin 13 effects on TF appear modulated by the activation of the G-protein-transcription factor nuclear factor (NF)-ΚB axis since G-protein inhibitors suppressed NF-ΚB mediated TF expression. Data of the present study, although in vitro, indicate that apelin-13, induces a procoagulant phenotype in HUVECs and monocytes by promoting TF expression. These observations support the hypothesis that this adipokine might play a relevant role as an active partaker in athero-thrombotic disease.
- Subjects :
- 0301 basic medicine
Vasodilation
Atherothrombosis
Cell Separation
030204 cardiovascular system & hematology
Monocyte
Monocytes
0302 clinical medicine
Intercellular Signaling Peptides and Protein
Protein Isoforms
Endothelial Cell
Medicine (all)
NF-kappa B
Atherothrombosi
Hematology
Flow Cytometry
Phenotype
Apelin
Intercellular Signaling Peptides and Proteins
GTP-Binding Protein
Human
Signal Transduction
medicine.medical_specialty
Coronary Thrombosi
Human Umbilical Vein Endothelial Cell
Adipokine
Real-Time Polymerase Chain Reaction
Thromboplastin
03 medical and health sciences
Tissue factor
Adipokines
GTP-Binding Proteins
Internal medicine
medicine
Human Umbilical Vein Endothelial Cells
Humans
Secretion
RNA, Messenger
Messenger RNA
business.industry
Coronary Thrombosis
Endothelial Cells
Protein Isoform
In vitro
030104 developmental biology
Endocrinology
Gene Expression Regulation
Adipokines, atherothrombosis, tissue factor
Endothelium, Vascular
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Thrombosis and haemostasis 113 (2015): 363–372. doi:10.1160/TH14-05-0451, info:cnr-pdr/source/autori:Cirillo P.; Ziviello F.; Pellegrino G.; Conte S.; Cimmino G.; Giaquinto A.; Pacifico F.; Leonardi A.; Golino P.; Trimarco B./titolo:The adipokine apelin-13 induces expression of prothrombotic tissue factor/doi:10.1160%2FTH14-05-0451/rivista:Thrombosis and haemostasis/anno:2015/pagina_da:363/pagina_a:372/intervallo_pagine:363–372/volume:113, Thrombosis and haemostasis (2014)., info:cnr-pdr/source/autori:Cirillo P1, Ziviello F, Pellegrino G, Conte S, Cimmino G, Giaquinto A, Pacifico F, Leonardi A, Golino P, Trimarco B./titolo:The adipokine apelin-13 induces expression of prothrombotic tissue factor./doi:/rivista:Thrombosis and haemostasis/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Accession number :
- edsair.doi.dedup.....ccf8b1fd06531fb321be5a42f1be87e7
- Full Text :
- https://doi.org/10.1160/TH14-05-0451