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Differences between Platelets Derived from Neonatal Cord Blood and Adult Peripheral Blood Assessed by Mass Spectrometry

Authors :
Stokhuijzen, Eva
Koornneef, Johanna M
Nota, Benjamin
van den Eshof, Bart Laurens
van Alphen, Floris Pieter Joachim
van den Biggelaar, Maartje
Van Der Zwaan, Carmen
Kuijk, Carlijn
Mertens, Koen
Fijnvandraat, Karin
Meijer, Alexander Benjamin
Afd Pharmaceutics
Pharmaceutics
Biomolecular Mass Spectrometry and Proteomics
Graduate School
ARD - Amsterdam Reproduction and Development
ACS - Amsterdam Cardiovascular Sciences
Paediatric Infectious Diseases / Rheumatology / Immunology
ACS - Pulmonary hypertension & thrombosis
Afd Pharmaceutics
Pharmaceutics
Biomolecular Mass Spectrometry and Proteomics
Source :
Journal of Proteome Research, 16(10), 3567. American Chemical Society, Journal of proteome research, 16(10), 3567-3575. American Chemical Society
Publication Year :
2017
Publisher :
American Chemical Society (ACS), 2017.

Abstract

It has been proposed that differences may exist between umbilical cord blood (CB) platelets and adult peripheral blood (APB) platelets, including altered protein levels of the main platelet integrins. We have now compared the protein expression profiles of CB and APB platelets employing a label-free comparative proteomics approach. Aggregation studies showed that CB platelets effectively aggregate in the presence of thromboxane A2 analogue, collagen, and peptide agonists of the proteinase-activated receptors 1 and 4. In agreement with previous studies, higher concentrations of the agonists were required to initiate aggregation in the CB platelets. Mass spectrometry analysis revealed no significant difference in the expression levels of critical platelet receptors like glycoprotein (GP)Ib, GPV, GPIX, and integrin αIIbβ3. This was confirmed using flow cytometry-based approaches. Gene ontology enrichment analysis revealed that elevated proteins in CB platelets were in particular enriched in proteins contributing to mitochondrial energy metabolism processes. The reduced proteins were enriched in proteins involved in, among others, platelet degranulation and activation. In conclusion, this study reveals that the CB and APB platelets are distinct. In particular, changes were observed for proteins that belong to metabolic and energy generation processes and not for the critical adhesive platelet integrins and glycoproteins.

Details

ISSN :
15353907 and 15353893
Volume :
16
Database :
OpenAIRE
Journal :
Journal of Proteome Research
Accession number :
edsair.doi.dedup.....cd227a444bcb1091acfb28800e45f753