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In vivo evidence for the involvement of anionic phospholipids in initiation of DNA replication in Escherichia coli
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 92(3)
- Publication Year :
- 1995
-
Abstract
- In vitro, anionic phospholipids can reactivate inactivated DnaA protein, which is essential for initiation of DNA replication at the oriC site of Escherichia coli [Sekimizu, K. & Kornberg, A. (1988) J. Biol. Chem. 263, 7131-7135]. Mutations in the pgsA gene (encoding phosphatidylglycerophosphate synthase) limit the synthesis of the major anionic phospholipids and lead to arrest of cell growth. We report herein that a mutation in the rnhA gene (encoding RNase H) that bypasses the need for the DnaA protein through induction of constitutive stable DNA replication [Kogoma, T. & von Meyenburg, K. (1983) EMBO J. 2, 463-468] also suppressed the growth arrest phenotype of a pgsA mutant. The maintenance of plasmids dependent on an oriC site for replication, and therefore DnaA protein, was also compromised under conditions of limiting anionic phospholipid synthesis. These results provide support for the involvement of anionic phospholipids in normal initiation of DNA replication at oriC in vivo by the DnaA protein.
- Subjects :
- Anions
DNA Replication
Ribonuclease H
Replication Origin
Biology
Pre-replication complex
Origin of replication
Replication factor C
Suppression, Genetic
SeqA protein domain
Bacterial Proteins
Escherichia coli
Phospholipids
Multidisciplinary
Models, Genetic
Phosphotransferases
DNA replication
DnaA
DNA-Binding Proteins
Biochemistry
Prokaryotic DNA replication
Genes, Bacterial
Mutation
Origin recognition complex
bacteria
Plasmids
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 92
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....cd5500a2d5f32b4641ab684237aeb3e4