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Characterization of a protein recognizing minor groove binders-damaged DNA
- Source :
- Nucleic Acids Research. 24:4227-4233
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- By using electromobility shift assay (EMSA), we have identified a protein able to recognize the DNA only if it was previously reacted with minor groove binders. This protein binds with very high affinity AT containing DNA treated with minor groove binders such as distamycin A, Hoechst 33258 and 33342, CC-1065 and ethidium bromide minor groove intercalator, but not with major groove binders such as quinacrine mustard, cisplatin or melphalan, or with topoisomerase I inhibitor camptothecin or topoisomerase II inhibitor doxorubicin. This protein was found to be present in different extracts of human, murine and hamster cells, with the human protein which appears to have a molecular weight slightly lower than that of the other species. This protein was found to be expressed both in cancer and normal tissues. By using molecular ultrafiltration techniques as well as southwestern analysis it was estimated that the apparent molecular weight is close to 100 kDa. We can exclude an identity between this protein and other proteins, with a similar molecular weight previously reported to be involved in DNA damage recognition/repair, such as topoisomerase I, mismatch repair activities such as the prokaryotic MutS protein and its human homologue hMSH2 or proteins of the nucleotide excision repair system such as ERCC1, -2, -3 and -4.
- Subjects :
- DNA damage
Antineoplastic Agents
Biology
Topoisomerase-I Inhibitor
DNA-binding protein
Cell Line
Jurkat Cells
Mice
chemistry.chemical_compound
Cricetinae
Tumor Cells, Cultured
Genetics
Animals
Humans
Electrophoretic mobility shift assay
Melphalan
Binding Sites
Topoisomerase
Distamycins
DNA
Molecular biology
Intercalating Agents
DNA-Binding Proteins
Mice, Inbred C57BL
Biochemistry
chemistry
Nitrogen Mustard Compounds
Bisbenzimidazole
biology.protein
Electrophoresis, Polyacrylamide Gel
Topoisomerase-II Inhibitor
Research Article
DNA Damage
Nucleotide excision repair
Subjects
Details
- ISSN :
- 13624962
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....cd7cf6e2102ccc7ca8285165baeaba39