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Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance
- Source :
- Plant Molecular Biology 86 (2014) 6, Plant Molecular Biology, 86(6), 641-653
- Publication Year :
- 2014
-
Abstract
- Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.
- Subjects :
- Arabidopsis
Pseudomonas syringae
Plant Science
transcription factors hahb1
Plant Epidermis
Laboratorium voor Plantenveredeling
Gene Expression Regulation, Plant
PRI Biodiversiteit en Veredeling
Disease Resistance
Oligonucleotide Array Sequence Analysis
Genetics
food and beverages
General Medicine
Plants, Genetically Modified
Phenotype
powdery mildew resistance
Oomycetes
expression patterns
vegetative storage protein
oidium-neolycopersici
PBR Biodiversity and genetic variation
Powdery mildew
Signal Transduction
Biology
Plant disease resistance
PBR Biodiversiteit en Genetische Variatie
Ascomycota
Stress, Physiological
plant defense
Animals
nudix hydrolase
Plant Diseases
Homeodomain Proteins
PBR Resistance in Solanaceae
Leucine Zippers
Hyaloperonospora arabidopsidis
Arabidopsis Proteins
Abiotic stress
Gene Expression Profiling
Biotic stress
biology.organism_classification
PBR Breeding for Resistance in Solanaceae
cell-death
Plant Leaves
PRI Biodiversity and Breeding
Mutagenesis, Insertional
Plant Breeding
salicylic-acid
Aphids
Downy mildew
EPS
Agronomy and Crop Science
hd-zip
Subjects
Details
- Language :
- English
- ISSN :
- 01674412
- Volume :
- 86
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Plant Molecular Biology
- Accession number :
- edsair.doi.dedup.....cdacad7d758b2774f024af07b640eeb3