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Modulation of phenotype and function of dendritic cells by a therapeutic synthetic killer peptide

Modulation of phenotype and function of dendritic cells by a therapeutic synthetic killer peptide

Authors :
Antonella Mencacci
Francesco Bistoni
Walter Magliani
Eva Pericolini
Anna Vecchiarelli
Luciano Polonelli
Stefania Conti
Elio Cenci
Source :
Journal of Leukocyte Biology. 79:40-45
Publication Year :
2005
Publisher :
Oxford University Press (OUP), 2005.

Abstract

The strong microbicidal effects of an engineered synthetic killer peptide (KP), which functionally mimics a fungal killer toxin, have been demonstrated extensively. β-glucan has been identified as a receptor for KP on fungal cell walls. Although the direct microbicidal and related therapeutic effects have been studied in depth, no information currently exists about the interaction of KP with immune cells. In this study, we exploited the possibility of KP binding to different murine immune cell populations. The results demonstrate that KP binds selectively to dendritic cells (DC) and to a lesser extent, to macrophages but not to lymphocytes and neutrophils; KP binding possibly occurs through major histocompatibility complex (MHC) class II, CD16/32, and cellular molecules recognized by anti-specific intercellular adhesion molecule-grabbing nonintegrin R1 antibodies; and KP modulates the expression of costimulatory and MHC molecules on DC and improves their capacity to induce lymphocyte proliferation. These findings provide evidence that this synthetic KP interacts selectively with DC and modulating their multiple functions, might also serve to improve the immune antimicrobial response.

Details

ISSN :
19383673 and 07415400
Volume :
79
Database :
OpenAIRE
Journal :
Journal of Leukocyte Biology
Accession number :
edsair.doi.dedup.....cdf86e1435015cb856b3762ea4921acf
Full Text :
https://doi.org/10.1189/jlb.0205113