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Ionic strength controls long-term cell-surface interactions – A QCM-D study of S. cerevisiae adhesion, retention and detachment
- Source :
- Journal of colloid and interface science
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Understanding microbial adhesion and retention is crucial for controlling many processes, including biofilm formation, antimicrobial therapy as well as cell sorting and cell detection platforms. Cell detachment is inextricably linked to cell adhesion and retention and plays an important part in the mechanisms involved in these processes. Physico-chemical and biological forces play a crucial role in microbial adhesion interactions and altering the medium ionic strength offers a potential means for modulating these interactions. Real-time studies on the effect of ionic strength on microbial adhesion are often limited to short-term bacterial adhesion. Therefore, there is a need, not only for long-term bacterial adhesion studies, but also for similar studies focusing on eukaryotic microbes, such as yeast. Hereby, we monitored, in real-time, S. cerevisiae adhesion on gold and silica as examples of surfaces with different surface charge properties to disclose long-term adhesion, retention and detachment as a function of ionic strength using quartz crystal microbalance with dissipation monitoring. Our results show that short- and long-term cell adhesion levels in terms of mass-loading increase with increasing ionic strength, while cells dispersed in a medium of higher ionic strength experience longer retention and detachment times. The positive correlation between the cell zeta potential and ionic strength suggests that zeta potential plays a role on cell retention and detachment. These trends are similar for measurements on silica and gold, with shorter retention and detachment times for silica due to strong short-range repulsions originating from a high electron-donicity. Furthermore, the results are comparable with measurements in standard yeast culture medium, implying that the overall effect of ionic strength applies for cells in nutrient-rich and nutrient-deficient media.<br />SCOPUS: ar.j<br />info:eu-repo/semantics/published
- Subjects :
- Surface Properties
Physique de l'état solide
Saccharomyces cerevisiae
QCM-D
02 engineering and technology
010402 general chemistry
01 natural sciences
Bacterial Adhesion
Biomaterials
Colloid and Surface Chemistry
Chimie des colloïdes
Zeta potential
XDLVO theory
Surface charge
Cell detachment
Biofilm formation
Cell adhesion
Cell retention
Chemistry
Osmolar Concentration
Biofilm
Chimie des surfaces et des interfaces
Métallurgie
Adhesion
Quartz crystal microbalance
Cell sorting
021001 nanoscience & nanotechnology
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Ionic strength
Quartz Crystal Microbalance Techniques
Biophysics
0210 nano-technology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Journal of colloid and interface science
- Accession number :
- edsair.doi.dedup.....ce05c06ee6b4ab782e0cba73570414df